UPLC法测定同仁乌鸡白凤丸中芍药苷的含量  

UPLC determination of paeoniflorin in Tongren Wuji Baifeng pills

作　　者：姚令文[1] 陈佳[1] 王钢力[1] 林瑞超[1] 

机构地区：[1]中国药品生物制品检定所,北京100050

出　　处：《药物分析杂志》2009年第9期1522-1524,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:用超高效液相色谱法测定同仁乌鸡白凤丸中芍药苷的含量。方法:参考已有文献方法对原高效液相色谱方法进行转换并优化为超高效液相色谱方法。色谱柱为Waters ACQUITY BEH C18柱(2.1mm×50mm,1.7μm);流动相:甲醇-水(20∶80);流速:0.4mL·min-1;检测波长:232nm;柱温:35℃。结果:芍药苷的线性范围为9.92～99.2ng(r=0.9999);平均回收率(n=6)为102.0%,RSD为2.0%;1个分析流程大约需要5min。结论:与高效液相色谱法比较,超高效液相色谱法测定同仁乌鸡白凤丸中芍药苷含量,既加快了分析速度,达到了提高分析效率,节约分析时间的目的,减少了有机溶剂的使用,又得到了更高的分析灵敏度。Objective：To develop an ultra -performance liquid chromatography （UPLC） method for the determina- tion of paeoniftorin in Tongren Wuji Baifeng pills. Methods： A Waters ACQUITY UPLCTM BEH C18column（2. 1 mm × 50 mm, 1.7 μm） was adopted with the mobile phase of methanol - water （ 20： 80） at a flow rate of 0. 4 mL · min-1. The detecting wavelength was set at 232 nm, and the column temperature was 35 ℃. Results： The calibra- tion curve of paeoniflorin was linear in the range of 9. 92 - 99. 2 ng （ r = 0. 9999 ） , the mean recovery （ n = 6） was 102.0% with RSD 2.0% , the time for a analysis program was about 5 minutes. Conclusion： Comparing with the HPLC method, the UPLC method for determination of paeoniflorin in Tongren Wuji Baifeng pills increases the anal- ysis speed and efficiency;it can also increase the analysis sensitivity and save the solvents.

关 键 词：超高效液相色谱 芍药苷 同仁乌鸡白凤丸 

分 类 号：R917[医药卫生—药物分析学]