HPLC测定当归补血片中黄芪甲苷的含量  

HPLC determination of astragaloside Ⅳ in Danggui Buxue tablets

作　　者：刘玮锦[1] 蔡大可[1] 刘亮锋[1] 赖平[1] 董婷霞[2] 詹华强[2] 苏子仁[1] 

机构地区：[1]广州中医药大学,广州510405 [2]香港科技大学

出　　处：《药物分析杂志》2009年第9期1553-1555,共3页Chinese Journal of Pharmaceutical Analysis

摘　　要：目的:建立当归补血片中黄芪甲苷的含量测定方法。方法:以吡啶-苯甲酰氯(2.5∶1)为衍生化试剂,对黄芪甲苷进行苯甲酰化,采用HPLC测定样品中黄芪甲苷含量。色谱柱:Phenomenex Gemini C18柱(50mm×4.6mm,5μm);流动相:乙腈-0.1%三乙胺溶液(98∶2)洗脱30min;流速1.0mL·min-1;检测波长230nm;柱温20℃;进样量10μL。结果:黄芪甲苷进样量在0.074～0.925μg范围内线性关系良好(r=0.9999),重复性好(RSD=0.17%),平均回收率(n=3)为98.6%～99.9%(RSD=0.32%～0.95%)。结论:该法样品前处理简单,专属性强、准确度高、灵敏度高、重现性好。Objective： To establish a method for the determination of astragaloside IV in Danggui Buxue tablets. Methods： Astragaloside IV in Danggui Buxue tablets was determined by HPLC with pre - column derivat- ization, the derivatizing reagent were benzoyl chloride and pyridine. Column： Phenomenex Gemini C^s column （50 mm x 4. 6 mm, 5 I^m） ; mobile phase ： acetonitrile - 0. 1% triethylamine solution （ 98： 2 ） with the same gradient elution of 30 rain at the flow rate of 1.0 mL · min -1 ;detection wavelength：230 nm;column temperature：20 ℃ ; injection volume ： 10 μL. Results ： The linear range of astragaloside IV was 0. 074 - 0. 925 μg（ r = 0. 9999 ） ; The method had a good repeatability（ RSD =0.17% ） and average recovery（98.6% -99.9% ） with RSD of 0.32% - 0.95 %. Conclusion： The method is accurate, specific, sensitive and reproducible, and preparation of the sam- ple is simple.

关 键 词：当归补血片 黄芪甲苷 衍生化 HPLC 

分 类 号：R917[医药卫生—药物分析学]