参芎滴丸预处理对大脑中动脉闭塞模型大鼠脑梗死体积、尼氏体的研究  

The effect of preconditioning with Shenxiong Pill on the infarct volumes and nissle bodies in SD rats by middle cerebral artery occlusion (MCAO)

作　　者：金硕果[1] 陈卫银[2] 刘福友[2] 黄梅 朱观祥[4] 王惠民[5] 

机构地区：[1]成都中医药大学临床医学院,四川成都610075 [2]成都中医药大学附属医院,神经内科,四川成都610072 [3]南充市中心医院中医科,四川南充637000 [4]浙江绍兴市中医院神经内科,浙江绍兴312000 [5]天津市南开医院中西医结合脑病中心,天津300100

出　　处：《中国中医基础医学杂志》2009年第7期501-503,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：四川省教育厅自然科学科研重点项目(2003A043);四川省中医管理局科研课题(2003A23)

摘　　要：目的:观察益气活血复方参芎滴丸预处理对局灶性脑缺血模型SD大鼠脑梗死体积、尼氏体的变化,探讨其在诱导脑缺血耐受中的意义。方法:电凝法制作大脑中动脉闭塞大鼠模型,造模前按5、10、20倍临床剂量灌胃8d,与环磷酰胺作对照。用图像处理软件计算脑梗死体积、光镜下观察尼氏体结构、采用Mias-2000高清晰度彩色病理图文报告系统半定量分析尼氏体染色。结果:与模型组比较,参芎滴丸高、中、低剂量组及环磷酰胺组72h梗死体积明显缩小(P<0.05);参芎滴丸3个剂量组均能增加尼氏体的表达,3组之间有量效关系(P<0.05)。结论:参芎滴丸预处理对局灶性脑缺血模型SD大鼠有神经保护作用,可诱导脑缺血耐受。Objective： To investigate the effect of preconditioning with Shenxiong Pill on the infarct volumes and nissle bodies in rats with middle cerebral artery occlusion （MCAO）, to explore Shenxiong Pill＇s role in ischemic tolerance in SD rats. Methods： Before rats with MCAO by electrocoagulation were made, the Shenxiong Pill was given for 8 days through stomach, and the control group was given by cyclophosphamide. Infarct volumes and nissle bodies were measured with Computer-assisted imaging and Optics microscope Results ： Compared with the model group, Infarct volumes in the groups of Shenxiong Pill and cyclophosphamide decreased obviously（ P 〈 0.05）, and also Shenxiong Pill could improve the expression of nissle bodies（ P 〈 0.05）. Conclusion：Preconditioning with Shenxiong Pill had the protective effect to the brain of SD rats with MCAO and could induce ischemlc tolerance.

关 键 词：益气活血 预处理 局灶性脑缺血模型 尼氏体 缺血耐受 

分 类 号：R285.5[医药卫生—中药学]