扶正解毒汤对镍诱导NRK细胞微核形成的抑制作用  

Inhibitory Effect of Fuzheng Jiedu Decoction on Micronuclei Formation Induced by Nickel Sulfate in NRK Cells

作　　者：滕玉莲[1] 吕晓云[2] 朱玉真[2] 赵健雄[2] 王敏[2] 

机构地区：[1]兰州大学第一医院,甘肃兰州730000 [2]兰州大学中西医结合研究所,甘肃兰州730000

出　　处：《中国中医基础医学杂志》2009年第8期591-592,595,共3页JOURNAL OF BASIC CHINESE MEDICINE

基　　金：教育部博士点专项科研基金(20050730031)

摘　　要：目的:研究扶正解毒汤(FJD)对硫酸镍(NiSO4)诱导大鼠肾细胞系(NRK)微核形成的抑制作用。方法:以NiSO4、FJD含药血清处理NRK细胞,检测微核率的变化及细胞总的清除自由基抗氧化力(TRAC)。结果:NiSO4(400μmol/L)暴露组细胞微核率、TRAC明显下降,低、中、高剂量FJD血清与NiSO4共处理组细胞微核率显著低于NiSO4暴露组(P<0.05,P<0.01),细胞TRAC则显著提升(P<0.05,P<0.01)。结论:NiSO4可诱发细胞微核形成,FJD对镍诱导NRK细胞微核形成有明显抑制作用,其机制可能与抗氧化应激有关。Objectives： To study the inhibitory effect of Fuzheng Jiedu Decoction（FJD） on the micronuclei formation induced by nickel sulfate（NiSO4） in rat renal cell line（NRK）.Methods：Cultured NRK cells were exposed to NiSO4（400μmol/L） and FJD drug serum.Micronuclei（MN） were scored in 1000 NRK cells and cellar total radical-trapping antioxidative capacity（TRAC）was measured.Results： Compared with NiSO4 group,the MN frequencies decreased and TRAC increased in groups treated with both FJD and NiSO4 significantly （ P 〈 0.05, P 〈 0.01 ）. Conclusion ： FJD could suppress nickel-induced micronuclei formation in NRK ceils which was probably related to its antioxidant property.

关 键 词：扶正解毒汤 硫酸镍 微核 肾细胞 总抗氧化力 

分 类 号：R285.5[医药卫生—中药学]