血红蛋白氧合酶-1抑制剂锌原卟啉对高体积分数氧肺损伤早产大鼠的影响  

Influence of Heme Oxygenase-1 Inhibitor Protoporphyrin Ⅸ Zinc on Hyperoxic Lung Injury in Preterm Rats

作　　者：全裕凤[1] 郑明慈[2] 张华[1] 张培林[1] 张红[1] 

机构地区：[1]桂林医学院附属医院新生儿科,广西桂林541001 [2]桂林医学院附属医院围产医学研究室,广西桂林541001

出　　处：《实用儿科临床杂志》2009年第18期1409-1411,共3页Journal of Applied Clinical Pediatrics

基　　金：广西科技厅科技攻关基金项目资助(0472002-35)

摘　　要：目的探讨锌原卟啉(Znpp)对高体积分数氧(高氧)肺损伤早产大鼠的影响。方法出生3 d SD早产大鼠随机分为空气对照组(Ⅰ组),高氧对照组(Ⅱ组),空气加Znpp组(Ⅲ组),高氧加Znpp组(Ⅳ组)。Ⅱ组、Ⅳ组吸入氧体积分数≥900 mL/L,Ⅲ组和Ⅳ组每日腹腔注射Znpp 45μmol/(kg.d)。于实验第3、7天,分别检测其肺组织匀浆血红蛋白氧合酶-1(HO-1)活性、碳氧血红蛋白(HbCO)水平及支气管肺泡灌洗液TNF-α、总蛋白、丙二醛(MDA)水平,测定肺湿/干质量比值(W/D),光镜下观察其肺组织病理学变化。结果实验第3天,Ⅱ组早产大鼠HO-1活性、HbCO水平、肺W/D及TNF-α水平、总蛋白、MDA水平明显高于Ⅰ组(Pa<0.05,0.01);Ⅳ组HO-1活性、HbCO水平、肺W/D及TNF-α水平、MDA水平明显低于Ⅱ组(Pa<0.01,0.05);实验第7天,Ⅱ组HO-1活性、HbCO水平及TNF-α水平、总蛋白、MDA水平明显高于Ⅰ组(Pa<0.01);Ⅳ组HO-1活性、HbCO水平及TNF-α水平、总蛋白、MDA水平明显低于Ⅱ组(Pa<0.01,0.05)。第3天,Ⅱ组肺组织呈急性炎性反应性改变,Ⅳ组炎性反应减轻明显;第7天,Ⅱ组呈亚急性炎性反应性改变、肺间质纤维化、肺泡发育障碍等,Ⅳ组病理损害有所减轻。结论HO-1参与早产大鼠高氧肺损伤。Znpp可通过抑制HO-1活性,减轻早产鼠高氧肺损伤。Objective To explore the effect of protoporphyrin IX zinc （Znpp） on hyperoxic lung injury in preterm rats. Methods Three - day - old preterm SD rats were randomly assigned to room air control group （group Ⅰ ）hyperoxia control group （oxygen≥ 900 mL/L） （group Ⅱ） , room air plus Znpp group（ group Ⅲ ） , hyperoxia plus Znpp group （group Ⅳ ）. Group Ⅲ and group Ⅳ were injected intraperitoneally with ZnPP 45 μmol/kg each day. After the third day and the 7th day of exposure, the activity of heme oxygenase - 1 （ HO - 1 ） and the percent of carboxyhemoglobin （HbCO） in the lungs, the lung wet weight/dry weight ratio （W/D）, tumor necrosis factor - α （TNF - α ）, total protein and malondialdehyde （MDA） in bronchoalveolar lavage fluid were determined and lung histophathological changes were examined in all groups. Results On the third day,compared with group I the activity of HO - 1 and the percent of HbCO in the lungs,W/D,TNF α, total protein and MDA, all greatly rised in group Ⅱ （Pa 〈0.05,0.01 ,respectively） ,and the activity of HO- 1 and the percent of HbCO in the lungs, W/D, TNF - α, MDA in group Ⅳ significantly dropped compared with those in group Ⅱ （Pa 〈 0. 01,0.05, respectively） ; On the 7^th day, the activity of HO - 1 and the percent of HbCO in the lungs, TNF - α, total protein, MDA in group lI all went up markedly compared with those in group Ⅰ （Pa 〈0.01 ） ,and the activity of HO - 1 ,the percent of HbCO in the lungs,TNF - α,total protein,MDA in group Ⅳ all significantly reduced compared with those in group Ⅱ （Pa〈 0.01,0.05, respectively）. Group 11 showed acute lung injury characterized by the presence of hyperaemia, red cell extravasation,and inflammatory infiltration after 3 days, the pulmonary pathologic changes alleviated in group Ⅳ compared with that in group Ⅱ. After 7 days of exposure, Group Ⅱ showed subacnte inflammatory response, pulmonary fibrosis, alveolar stunting, while the changes were relieved in gr

关 键 词：血红蛋白氧合酶 高体积分数氧 肺 锌原卟啉 大鼠 早产 

分 类 号：R722.6[医药卫生—儿科]