橡胶树枝条内生真菌的分离及其拮抗性的测定  

Isolation and Identification of Endophytic Fungi from Rubber Tree and Their Antagonism to Plant Pathogens

作　　者：郑鹏[1] 何静[1] 常凯军[1] 张盛敏[1] 谭德冠[1] 孙雪飘[1] 张家明[1] 

机构地区：[1]中国热带农业科学院热带生物技术研究所农业部热带作物生物技术重点实验室,海口571101

出　　处：《热带作物学报》2009年第6期832-837,共6页Chinese Journal of Tropical Crops

基　　金：中央级公益性科研院所基本科研业务费专项资金资助项目(No.ITBBZD2007-1-1);海南省自然科学基金项目(No.309051)资助

摘　　要：经过严格的表面消毒程序,从橡胶树枝条中分离到18株内生真菌,并对其进行初步鉴定。结果表明,这18株内生真菌分别归属于2个目,2个科,7个属,其优势菌群为无孢菌群(Mycelia Sterilia)。通过温度测定,确定橡胶树内生真菌最适生长温度为25～28℃;采用平板对峙培养法,研究内生真菌对香蕉枯萎病菌(Fusarium oxysporumf.sp.cubense)和橡胶炭疽病菌(Colletotrichum gloeosporioides Penz.Sace)的抑菌作用。结果表明,ITBB2-2对两种植物病原菌的拮抗作用最好,ITBB2-10和ITBB2-15次之,ITBB2-3对香蕉枯萎病菌拮抗作用明显而对橡胶炭疽病菌拮抗作用不明显,其它内生真菌对两种植物病原菌无拮抗作用或不明显。Eighteen endophytic fungal strains were isolated from rubber trees（Hevea brasiliensis） after strict surface sterilization. These 18 fungi belonged to 7 genera under 2 families and 2 orders based on morphological characteristics. Of the fungi isolated mycelia sterilia were dominant. The optimum temperature for growth of these endophytic fungi ranged from 25 ℃ to 28 ℃. All the isolated fungi were tested in agar-plate duel culture to observe their inhibitory activities against Fusarium oxysporum f. sp. cubense and Colletotrichum gloeosporioides Penz.Sace. Results showed that Strain ITBB2-2 had the highest antagonism against F.oxysporum f. sp. cubense and C. gloeosporioides, followed by Strains ITBB2-10 and ITBB2-15, and that Strain ITBB2-3 inhibited obviously F. oxysporum f. sp. cubense but not C. gloeosporioides. The other strains showed no or little inhibitory effect on the growth of the 2 plant pathogens tested.

关 键 词：橡胶树 内生真菌 香蕉枯萎病 橡胶炭疽病 拮抗作用 

分 类 号：S794.1[农业科学—林木遗传育种]