角化细胞生长因子对骨髓间充质干细胞体外诱导分化为肺泡上皮细胞的实验研究  

In vitro effect of Keratinocyte growth factor on the differentiation of bone marrow mesenchymal stem cells into lung alveolar epithelial cells

作　　者：王艳[1] 杨志军[2] 封志纯[2] 

机构地区：[1]南方医科大学珠江医院儿科,广东广州510080 [2]北京军区总医院附属八一儿童医院新生儿科

出　　处：《中国输血杂志》2009年第8期628-632,共5页Chinese Journal of Blood Transfusion

基　　金：国家自然科学基金资助课题(No.30772036)

摘　　要：目的观察角化细胞生长因子(KGF)对体外小鼠骨髓间充质干细胞(MSCs)向肺泡上皮细胞诱导分化的影响。方法建立体外非接触共培养诱导小鼠MSCs向肺泡上皮细胞分化的实验模型,并在培养液中加入KGF(10μl/ml),小鼠分4组(6只/组),实验组A:MSCs(1×105)单独培养组+KGF;实验组B:MSCs+正常肺组织悬液(1×105)+KGF;实验组C:MSCs+损伤肺组织悬液+KGF;实验组D:MSCs+损伤肺组织悬液(1×105)。共同培养8 d,应用激光共聚焦显微镜和RT-PCR检测共培养体系下室MSCs中的SP-C和AQP5的表达情况。结果实验组C于共培养的d 8,较其它实验组的AQP5 mRNA的表达量明显增多(5550.00±244.39vs1650.17±184.02,1600.83±193.00,2890.00±179.09,P均<0.05);于共培养的d 5,与实验组A相比差异也有统计学意义(1743.17±228.41vs1482.00±156.11,P<0.05)。只有实验组C和D表达SP-C,且实验组C各培养时间点的SP-C的mRNA的表达量均较实验组D明显增多(2632.50±231.11vs1599.00±158.95;3976.67±221.81vs2066.83±101.31;5388.33±347.96vs3893.17±178.61,P均<0.01)。结论KGF可以进一步促进体外小鼠MSCs诱导分化为肺泡上皮细胞。Objective To observe the impact of keratinocyte growth factor （KGF） on the differentiation of bone marrow mesenchymal stem cells （MSCs） into lung alveolar epithelial cells in vitro. Methods The co-culture model of inducing Bone Marrow Mesenchymal Stem Cells （MSCs） to differentiate into lung alveolar epithelial cells was established, KGF （10ul/ml） was added into the medium. There were 4 groups, each consisting 6 samples： group A was MSCs （ 1×10^5 ） alone; group B was MSCs cultured with cells from normal lung tissue（1×10^5） ; and group C and D were MSCs with cells from injured lung tissue（1×10^5）, the group D had no KGF. Each group was cultured for 8 days and AQP5 and SP-C, the two markers of lung alveolar epithelial cells were measured by laser confocal microscopy and RT-PCR. Results The AQP5 mRNA expression was significantly increased in group A on the 8th cultured day, compared with all other three groups （5 550.00 ±244. 39 vs 1 650. 17 ± 184. 02,1 600. 83 ± 193.00,2 890.00 ± 179. 09, P 〈 0. 05）. The AQP5 mRNA in group A was also significantly higher on the 8th day, compared to the same group on the 5th day （ 1 743. 17 ± 298. 41 vs 1 482. 00 ± 156. 11, P 〈0.05）. SP-C was only detected in the group C and D, and SP-C mRNA expression was significantly increased in group C compared with group D on the 1st, 5th and 8th cultured day （2 632. 50 ±231.11 vs 1 599. 00 ± 158.95 ;3 976.67 ± 221.81 vs 2 066. 83 ± 101.31 ;5 388. 33 ± 347.96 vs 3 893.17± 178. 61 ,P 〈 0.01 ）. Conclusion Keratinocyte Growth Factor could promote mouse MSCs to differentiate into the lung alveolar epithelial cells in the co-culture system with the cells from injured lung tissue.

关 键 词：骨髓间充质干细胞 肺泡上皮细胞 角化细胞生长因子(KGF) 激光共聚焦 

分 类 号：R725[医药卫生—儿科] R329[医药卫生—临床医学]