骨髓间充质干细胞诱导分化的成骨细胞转染增强型绿色荧光蛋白基因后复合生物衍生骨的研究  

Osteoblasts derived from human marrow mesenchymal stem cells on co-cultured tissue engineering bone after transfected by EGFP genes

作　　者：黄晓兵[1] 刘霆[2] 邓力[3] 智伟[3] 李秀群[2] 孟文彤[2] 

机构地区：[1]四川省医学科学院,四川省人民医院血液科,成都610072 [2]四川大学华西医院血液科 血液病研究室,成都610041 [3]四川大学华西医院干细胞与组织工程实验室,成都610041

出　　处：《南京医科大学学报（自然科学版）》2009年第10期1360-1364,1369,共6页Journal of Nanjing Medical University(Natural Sciences)

摘　　要：目的:探讨绿色荧光蛋白(GFP)基因转染本身是否可以改变细胞复合材料的生物学特性,从而影响材料生物相容性的正确评价。方法:将增强型绿色荧光蛋白(EGFP)转基因技术成功标记人源骨髓间充质干细胞(MSCs)诱导分化的成骨样细胞,再与人源生物衍生骨复合培养;同时设立未转染EGFP基因的骨髓MSCs诱导分化的成骨样细胞直接与人源生物衍生骨复合培养作为对照,通过倒置显微镜、荧光显微镜、扫描电镜对比观察。结果:发现转染EGFP基因的MSCs诱导分化的成骨样细胞能够成功地复合于人源生物衍生骨上,且生长状态较好。与未转染组细胞复合材料的情况相比,转染组与其没有明显差异。结论:EGFP基因转染技术没有对MSCs诱导分化的成骨样细胞复合衍生骨的能力以及增殖生长活性造成明显损害,从而不会影响对于材料生物相容性的正确评价。Objective：To explore the transfecting EGFP genes effect into cells on adherence ability of cells to biomaterials,which influences material biocompatibility evalution. Methods： EGFP gene was successflly delivered into osteoblasts induced from human marrow mesenchymal stem cells （MSCs） through electroporation transfection,and the osteblastes were seeded into tissue engineering bone with biomaterials. All cultured cells were detected by inverted microscope, scanning electron microscope, and fluorescent microscope. Results：It was observed that the osteoblasts transfected with EGFP gene had been successfully adhered to tissue engineering bone, and compared with osteoblasts without EGFP gene transfection, there was no obvious difference. Concluslon：It is suggested that transfection of GFP marker does not harm the biocompatibility to biomaterials and proliferative activity of osteoblasts induced from MSCs,which is helpful to understanding biocompatibility of materials accurately.

关 键 词：生物相容性 成骨细胞 增强型绿色荧光蛋白 组织工程人源生物衍生骨 

分 类 号：Q813.12[生物学—生物工程]