Expression of Hepatitis B Surface Antigen Gene in Ginseng Cells  

作　　者：YU Hai-peng XUE Yan AN Wei LIU Dan HAO Shu-mei SHENG Jun 

机构地区：[1]College of Life Science, Jilin University, Changchun 130012, P R. China [2]Changchun Institute of Biological Products, Changchun 130062, P R. China [3]Changchun University of Science and Technology, Changchun 130022, 13. R. China [4]Organ Transplantation Department of First Hospital, Jilin University, Changchun 130021, P R. China

出　　处：《Chemical Research in Chinese Universities》2009年第5期695-698,共4页高等学校化学研究（英文版）

基　　金：Supported by the Science and Technology Development Planning Foundation of Jilin Province of China(No.20030405)

摘　　要：The recombinant plasmid pBIBSa containing the HBsAg DNA fragment was transferred into Agrobacterium tumefaciens strain LBA4404 directly. Ginseng cells were transfected with A. tumefaciens carrying pBIBSa and the ginseng cell lines carrying HBsAg-S gene were obtained. The presence of target gene in the transfect cells was confirmed by PCR and RT-PCR. A clear band at the site of 700 bp was observed by agarose electrophoresis analysis of the samples containing the target gene. HBsAg expressed by the transgenic ginseng cells was detected by Western blot. Maximum expression levels of 184 ng HBsAg/g FW and 0. 009% of the total soluble proteins were observed by ELISA. HBsAg in ginseng cells was located both on the cell membrane and in the nuclei.The recombinant plasmid pBIBSa containing the HBsAg DNA fragment was transferred into Agrobacterium tumefaciens strain LBA4404 directly. Ginseng cells were transfected with A. tumefaciens carrying pBIBSa and the ginseng cell lines carrying HBsAg-S gene were obtained. The presence of target gene in the transfect cells was confirmed by PCR and RT-PCR. A clear band at the site of 700 bp was observed by agarose electrophoresis analysis of the samples containing the target gene. HBsAg expressed by the transgenic ginseng cells was detected by Western blot. Maximum expression levels of 184 ng HBsAg/g FW and 0. 009% of the total soluble proteins were observed by ELISA. HBsAg in ginseng cells was located both on the cell membrane and in the nuclei.

关 键 词：Transgenic cell HBSAG GINSENG 

分 类 号：Q78[生物学—分子生物学] S567.51[农业科学—中草药栽培]