大鼠海马锥体神经元的急性分离方法  

The Method of Acute Isolation of Rat Hippocampal Pyramidal Neuron

作　　者：冯冠青[1] 赵世刚[2] 杨雷[2] 贺秉军[3] 傅志燕[3] 

机构地区：[1]鄂尔多斯市中心医院神经内科,内蒙古鄂尔多斯017000 [2]内蒙古医学院附属医院神经内科,内蒙古呼和浩特010050 [3]南开大学生命科学院,天津300071

出　　处：《内蒙古医学杂志》2009年第8期912-914,共3页Inner Mongolia Medical Journal

摘　　要：目的：建立一种适用于膜片钳技术的大鼠海马锥体神经元急性分离方法,并对其离子通道进行描述。方法：采用酶加机械分离法制备10~12 d鼠龄的大鼠海马锥体神经元,用全细胞膜片钳技术测定其生理学特性。结果：分离出的神经元形态正常,有较长突起;用膜片钳技术证实,其保存了主要的离子通道活性。结论：建立一种简单快速的适用于膜片钳技术的大鼠海马锥体神经元急性分离方法。Objective：To develop a modified method of acute isolation of rat hippocampal pyramidal neu- ron, and observe its ion current with patch clamp. Methods： The hippocampal pyramidal neurons were obtained by a combination of mechanical and enzymatic means from 10 -- 12 day - old rats. Whole - cell patch clamp tech- nique was used to measure the electrophysiological properties of the neurons. Results： The neurons isolated in this way had regular configuration. The neuraxons of them were very long. With patch- clamp techniques, we found that the methods could save the activities of primary iro.n channels. Conclusion： A simple and fast method for acutely isolating rats hippocampal pyramidal neuron suitable for patch clamp is successfully found.

关 键 词：海马锥体神经元 急性分离 膜片钳 离子通道 

分 类 号：R338[医药卫生—人体生理学]