基因敲入和转基因小鼠前列腺癌模型的组织学比较研究  

Histopathology studies of transgenic and knock-in prostate cancer mouse models

作　　者：武国军[1] 李晓武[1] 朱李兵[1] 王禾[1] 袁建林[1] 于磊[1] 张更[1] 王立国[1] 刘贺亮[1] 

机构地区：[1]第四军医大学西京医院泌尿外科,陕西西安710032

出　　处：《中国现代医学杂志》2009年第17期2619-2622,共4页China Journal of Modern Medicine

基　　金：军队医药卫生"十一五"科研项目(No:06H040);第四军医大学优秀博士学位论文课题资助项目

摘　　要：目的利用与临床Gleason分级系统相近的小鼠组织学分级系统分析比较基因敲入(KIMAP)和转基因(TGMAP)小鼠前列腺癌模型。方法依照前列腺癌临床诊断的Gleason五级10分制的组织学分级和评分标准,建立了基因工程小鼠模型的分析标准。对96例KIMAP和44例TGMAP前列腺癌标本进行了分析,并与临床前列腺癌进行了比较。结果来源于TGMAP(n=前列腺癌44例/总数187例)和KIMAP(n=前列腺癌96例/总数169例)的前列腺癌小鼠表现了不同的组织学评分的分布(P=0.000)。KIMAP小鼠(52.1%)比TGMAP小鼠(25.0%)表现出更高的混合组织学评分率,更接近于临床平均值(50.0%),而TGMAP小鼠在所有年龄组均显示出不平衡的和随意的组织学评分分布。结论由于KIMAP模型成功地模仿了人类前列腺癌特征,在前列腺癌临床前期研究中具有潜在的应用价值。[ Objective ] This study used a new mouse histological grading system similar to the human Gleason grading system to measure and compare the new knock-in mouse adenocarcinoma prostate model （KIMAP） with transgenic mouse adenocarcinoma prostate model （TGMAP） and human prostate cancer （CAP）. [ Methods ] According to heterogeneity of the clinical standard for prostate cancer diagnosis, a close-to-human mouse standard for histological grading and scoring system, Gleason analogous grading system, was established in this study. 96 K/MAP and 44 TGMAP prostate cancer samples were measured and compared with human CaP. [Results] Mice with CaP from TGMAP （n =CaP 44/total 187） and K/MAP （n =CaP 96/total 169） models showed a different distribution of histologi- cal scores （P =0.000）. K/MAP mice showed higher percentage （52.1%） of compound histological score rate than TGMAP （25.0%）, but closer to the clinical average （50.0%）, while TGMAP mice revealed unbalanced and random score distribution in all age groups. [ Conclusion ] Due to the effective mimicry of human CaP tumors demonstrated by the KIMAP model, this study advocates its use in the clinical study of human CaP

关 键 词：前列腺 肿瘤 小鼠模型 

分 类 号：R392.11[医药卫生—免疫学] R-332[医药卫生—基础医学]