塞来昔布抑制顺铂致大鼠迟发性呕吐的作用及机制研究  

Mechanism of Antiemetic Effect of Celecoxib on Cisplatin-induced Delayed Emesis in Rats

作　　者：鞠传霞[1] 岳旺[1] 孙福生[2] 平藤雅彦 

机构地区：[1]青岛大学医学院,青岛市266012 [2]青岛市立医院,青岛市266011 [3]日本北海道医疗大学

出　　处：《中国药房》2009年第28期2174-2176,共3页China Pharmacy

基　　金：国家自然科学基金资助项目(30873108)

摘　　要：目的:研究选择性环氧合酶-2抑制剂塞来昔布对顺铂致大鼠迟发性呕吐的作用及机制。方法:将大鼠分为对照组、顺铂组和塞来昔布组(顺铂+塞来昔布),各组给予相应药物后观察大鼠摄食高岭土量;测定肠组织中5-羟色胺(5-HT)及其代谢产物5-羟基吲哚乙酸(5-HIAA)含量;测定色氨酸羟化酶(TPH)和单胺氧化酶(MAO)活性;观察小肠黏膜形态学改变。结果:与对照组比较,顺铂组摄食高岭土量明显增加(P<0·05),肠组织内TPH活性增强,MAO活性减弱,5-HT的含量增加(P<0·05)。与顺铂组比较,塞来昔布组摄食高岭土量明显降低,TPH酶活性及5-HT的含量也均降低(P<0·05),且小肠组织病变减轻。结论:塞来昔布可以抑制顺铂所致化疗性异食癖,提示其可能具有防治迟发性恶心、呕吐的作用。OBJECTIVE： To study the action mechanism and effect of celecoxib on cisplatin - induced delayed emesis in rats. METHODS： The rats were divided into control group, cisplatin group, and celecoxib group （cisplatin plus celecoxib） . Kaolin intake in the rats after administration of corresponding drugs was observed. Levels of 5- HT and its metabolite 5- HIAA in intestinal tissues, and the activities of tryptophan hydroxylase （TPH） and monoamine oxidase （MAO） were deter- mined, and the morphological changes of the intestinal tissues were observed. RESULTS： In cisplatin group compared with control group, the Kaolin intake increased significantly （P〈0.05）, TPH activity in the intestine increased, MAO activity decreased while the content of 5-HT increased （P 〈 0.05） . In celecoxib group compared with cisplatin group, kaolin intake decreased significantly, TPH activity and the content of 5-HT decreased as well （P〈0.05）, and the pathologic changes of intestinal tissue ameliorated. CONCLUSION： The inhibitory effect of celecoxib on cisplatin - induced chemotherapeutic allol- riopagia suggests its preventive and curative effects on delayed nausea and emesis.

关 键 词：顺铂 塞来昔布 大鼠 呕吐 5-羟色胺 色氨酸羟化酶 单胺氧化酶 

分 类 号：R965[医药卫生—药理学] R979.1[医药卫生—药学]