黄芩及其炮制品黄芩片的HPLC指纹图谱研究  

Study on the HPLC Fingerprint of Radix Scutellariae and Its Processed Pieces

作　　者：杨云[1] 肖功胜[1] 闻永举[2] 田润涛 

机构地区：[1]河南中医学院药学院,郑州450008 [2]江西宜春学院,江西宜春336000 [3]珠海科曼中药研究有限公司,广东珠海519085

出　　处：《中国现代应用药学》2009年第9期720-724,共5页Chinese Journal of Modern Applied Pharmacy

基　　金：河南省重大科技攻关(200422030700)

摘　　要：目的对黄芩药材及其炮制品黄芩片进行HPLC指纹图谱研究。方法选择HPLC-UV指纹图谱。色谱条件:Hypersil C18柱(200mm×5.0mm,5μm);流动相为甲醇-0.4%磷酸水溶液-乙腈线性梯度洗脱;流速:1.0mL·min-1;柱温28℃;检测波长277nm。结果10批不同产地正品黄芩进行规范炮制加工后得到的黄芩片指纹图谱无明显差异,14个不同产地黄芩药材的指纹图谱也具有极高的相似度。结论该方法重复性好,可为黄芩药材及其饮片的质量控制提供科学依据。OBJECTIVE To study the HPLC fingerprints of Radix Scutellariae and its processed pieces. METHODS Selecting HPLC-UV fingerprints. Using a Hypersil C18 column （200 mm× 5.0 mm, 5 μm）. The mobile phase was linear gradient elute with methanol-0.4% phosphoric acid-acetonitrile. Flow rate was 1.0 mL.min^-1. Column temperature was 28 ℃. Detection wavelength was set at 277 nm. RESULTS There was no evident difference among the fingerprints of 10 batches of Radix Scutellariae that were normatively manufactured and processed,and the similarity of 14 batches of Radix Scutellariae was very well. CONCLUSION This method recurs well, and can be used to provide scientific basis for Radix Scutellariae and its processed pieces quanlity control mode.

关 键 词：黄芩 HPLC指纹图谱 黄芩饮片 

分 类 号：R931.5[医药卫生—生药学]