绞股蓝总皂甙对兔动脉内皮损伤后内膜增殖及sis基因表达的影响  

Effects of gypenosides on intimal proliferation and expression of c-sis oncogene in rabbit iliac artery afterballoon deendothelialization

作　　者：侯晓平[1] 贾国良[1] 赵宽[2] 贾广兴[2] 笪冀平[2] 

机构地区：[1]第四军医大学西京医院心血管内科,西安710033 [2]空军总医院

出　　处：《第四军医大学学报》1998年第5期501-504,共4页Journal of the Fourth Military Medical University

摘　　要：目的：研究绞股蓝总皂甙（GPs）、曲匹地尔(trapidil)对兔髂动脉内皮损伤后内膜增殖的抑制作用，以及上述过程中sis基因表达的改变及药物的影响．方法：用Fogarty球囊导管制成兔髂动脉内皮损伤模型，分别给予（GPs，traPidil治疗，于不同时间点进行病理形态学检查、平滑肌细胞肌动蛋白（SMCsactin）免疫组化染色及sis基因原位杂交（ISHH）．结果：术后10d，28d损伤血管内膜明显增生、增厚，管腔狭窄，血管中膜及增生内膜有大量actin染色阳性细胞；ISHH显示sis基因mRNA明显表达；GPs，tra－Pidil可明显抑制损伤血管内膜的增生［内膜面积分别为（0．095±0．035）mm2，(0．065±0．014)mm2vs（0．184±0．063）mm2，P＜O．05]，但对sis基因表达无明显影响．结论：GPs对在体兔具有明显的抑制实验性动脉内皮损伤后内膜增殖的作用．Aim :To determine if gypenosides (GPs) could inhibit SMCs proliferation and influence sis gene expressionafter experimental artery deendothelialization. Methods:Both GPs and trapidil were administered before and afterballoon deendothelialization in rabbit iliac artery. Mor-phometry,SMCs actin immunohistochemical staining and sisgene in situ hybridization histochemistry (ISHH) were per-formed on the sections obtained from arterial segments. Re-sults: The intimal hyperplasia and luminal stenosis could befound in injured arteries 10 d, 28 d after balloon denuda-tion. The areas of neo-intima in GPs group and trapidilgroup were significantly smaller than those in control(0. 095±0. 035)mm2 and (0. 065 ± 0. 014)mm2 μs (0. 184 ±0. 063) mm2, respectively, P< 0. 05 ). Numbers of SMCsactin-positive staining cells appeared in neo-intima and me-dia in 10 d, 28 d after arterial injury. Remarkable expression of sis gene in media and neo-intima of injured arterieswas detected by ISHH in 3 d, 10 d, and 28 d after balloondenudation with intimal hyperplasia. GPs and trapidil hadno effects on expression of sis gene. Conclusions: The results suggest that GPs have obvious inhibiting effects on in-timal hyperplasia in rabbit iliac artery after balloon arterialdenudation.

关 键 词：绞股蓝总皂甙 血管内皮损伤 内膜增殖 药理实验 

分 类 号：R285.5[医药卫生—中药学] R961[医药卫生—中医学]