湘西盲高原鳅群体同工酶分析  

Isozyme analysis of Triplophysa xiangxiensis population.

作　　者：贺刚[1,2] 何力[1] 许映芳[1] 王雪光[3] 李晓莉[1] 张征[1] 

机构地区：[1]中国水产科学院长江水产研究所,湖北荆州434000 [2]江西省水产科学研究所,南昌330039 [3]中华人民共和国农业部渔业局,北京100026

出　　处：《生态学杂志》2009年第10期2037-2041,共5页Chinese Journal of Ecology

基　　金：国家科技支撑计划资助项目(2006BAD01A12)

摘　　要：采用聚丙烯酰胺凝胶电泳(PAGE)对洞穴鱼类——湘西盲高原鳅(Triplophysa xiangxiensis)群体遗传多样性进行了研究。对其肌肉、心脏、肝脏、脑、脾脏和肾脏等6种组织中的乳酸脱氢酶(LDH)、苹果酸脱氢酶(MDH)、苹果酸酶(ME)、酯酶(EST)、醇脱氢酶(ADH)、谷氨酸脱氢酶(GDH)和葡萄糖-6-磷酸脱氢酶(G-6-PDH)等7种同工酶进行分析,检测到26个座位,其中多态座位4个(Ldh-3、Ldh-4、s-Mdh、m-Mdh),多态座位比例为15.38%,观察杂合度0.0398,预期杂合度0.0411,Hardy-Weinberg遗传偏离指数(D)为-0.0316,存在杂合子缺失。结果表明,湘西盲高原鳅群体群体遗传多样性较低,纯合度较高。By using vertical polyacrylamide gel electrophoresis （PAGE）, the genetic diversity of Triplophysa xiangxiensis （cave fish） population was studied. A total of 26 loci were detected in 7 isozymes （LDH, MDH, ME, EST, ADH, GDH, and G-6-PDH） of six tissues （muscle, heart, liver, brain, spleen, and kidney）, among which, Ldh-3, Ldh-4, s-Mdh and m-Mdh were polymorphic, with the mean proportion of polymorphic loci being 15.38%. The observed heterozygosity was 0. 0398, expected heterozygosity was 0. 0411, and genetic deviation index （D value） was -0. 0316, suggesting that T. xiangxiensis population had a lower genetic diversity and a higher genetic purity.

关 键 词：湘西盲高原鳅 同工酶 群体遗传分析 

分 类 号：S917.4[农业科学—水产科学]