微卫星位点获取方法的研究进展  

Methods for obtaining microsatellite loci:A review.

作　　者：孙波[1] 鲍毅新[1] 赵庆洋[1] 张龙龙[1] 胡知渊[1] 

机构地区：[1]浙江师范大学生态研究所,浙江金华321004

出　　处：《生态学杂志》2009年第10期2130-2137,共8页Chinese Journal of Ecology

基　　金：浙江省自然科学基金资助项目(Y507080)

摘　　要：微卫星标记(simple sequence repeat,SSR)是进行分子遗传学研究的一种有效手段,并以其多态性高、信息含量大、保守性等特点成为最受人们欢迎的分子标记之一。但微卫星标记具有种族特异性,必须采用特异引物进行PCR检测,因而存在引物开发的问题。本文就筛选基因组文库法、微卫星富集法、数据库查找法、近缘物种筛选法、TOMMI法和FI-ASCO法等具有代表性的微卫星标记开发策略进行了综述,旨在为分子生态学研究过程中微卫星位点筛选方法的选择提供参考。Microsatellite marker or simple sequence repeat （SSR） is an effective means in mo- lecular genetics research, and the most popular molecular marker, because of its high polymor- phism, large information contents, and conservative characteristics. However, SSR is a kind of special primer marker, and it is necessary to know a species DNA sequence to design the primers for PCR testing, a problem limiting microsatellite primer development. This paper reviewed the development strategies of representative microsatellite markers, such as genomic library screening, SSR enrichment, database search, relative species selection, TOMMI, and FIASCO, aimed to provide references to the microsatellite loci screening in molecular ecology research.

关 键 词：微卫星位点 分离方法 数据库查找 近缘物种 基因组DNA 

分 类 号：S917.4[农业科学—水产科学]