双歧杆菌脂磷壁酸和总DNA对小鼠免疫功能的影响  

Effects of lipoteichoic acid and total DNA of Bifidobacteria on immunological function of mice

作　　者：穆小萍[1] 张德纯[2] 邓文喻[1] 赖卫明[1] 赖科峰[1] 

机构地区：[1]广东省妇幼保健院,广东广州510010 [2]重庆医科大学临床检验诊断学省部共建教育部重点实验室,重庆400016

出　　处：《中国微生态学杂志》2009年第9期804-805,808,共3页Chinese Journal of Microecology

摘　　要：目的比较双歧杆菌2种组分即双歧杆菌脂磷壁酸(LTA)和总DNA的免疫调节作用。方法分别提取和制备双歧杆菌LTA和总DNA。采用淋巴细胞转化法和溶血空斑法分别研究它们对小鼠细胞和体液免疫的调节作用。结果与对照组比较,双歧杆菌和总DNA对T细胞和B细胞都有明显的刺激作用(P<0.05或P<0.01),但是LTA的作用更强(P<0.01)。结论双歧杆菌细胞壁的LTA和细胞核的总DNA均具有免疫调节作用,但前者效能优于后者。Objective To compare the immunological effects of two kinds of components of Bifidobacterium bifi- dum such as lipoteichoic acid （LTA） and total DNA. Method LTA and total DNA of Bifidobacterium bifidum were extracted and prepared with chemical methods. Lymphocyte transforming assay and Plaque forming assay were used to evaluate the cellular and humoral immunoloregulation of above anaerobic preparations. Result Compared with the control, both LTA and total DNA of Bifutobacterium bifidum could stimulate significantly T cells and B cells （ P 〈 0.05 or P 〈 0.01 ）, but LTA was better than total DNA （P 〈 0.01 ）. Conclusion Both LTA and total DNA of Bifidobecterium bifidum can increase the immunomodulating effect, but the efficacy of the former is better than the latter.

关 键 词：双歧杆菌 脂磷壁酸 DNA 免疫功能 

分 类 号：R378.992[医药卫生—病原生物学]