肿瘤冷热交替治疗的生物学效应初步研究  

Biological Effects Study of Alternated Cooling and Heating Treatment

作　　者：许毳毳[1] 隗功华[2] 孙建奇[1] 孙晓光[2] 刘苹[1] 张爱丽[1] 

机构地区：[1]上海交通大学生物医学工程系Med-X研究院,上海200240 [2]上海交通大学医学院附属仁济医院核医学科,上海200217

出　　处：《中国医学物理学杂志》2009年第5期1438-1445,共8页Chinese Journal of Medical Physics

基　　金：国家自然基金重点项目(No.50436030);自然基金杰出青年项目(No.50725622);上海市工程技术研究中心项目(No.08DZ2211201)

摘　　要：目的:在细胞水平及较大动物水平上研究冷热交替治疗方法。由于肿瘤单冷、单热治疗具有一定局限性,如肿瘤边缘温度控制、热耐受、热抑制;冷冻损伤复发等。而已有研究证明冷热交替治疗在小鼠肿瘤血管损伤及引发机体免疫响应等方面优于现有任何一种热物理治疗方法。方法:首先利用转移性乳腺癌细胞系MDA-MB-231模型,比较了不同热物理刺激对细胞的杀伤效果,其次结合病理分析和影像学方法,建立新西兰大白兔VX2乳腺癌模型。结果:发现冷热交替比单冷,单热造成更严重的细胞损伤;初步验证冷热交替治疗荷兔乳腺癌具有较好疗效,3个月内未出现转移和复发。结论:冷热交替治疗是一种有效的肿瘤治疗手段。Objective： We put forward to study on the cellular level and a greater level of animal model, As the single treatment of cold or heat have some limitations, such as the edge temperature control of tumor, heat resistance, and post-thaw injury recurrence. It is proved that, the results in curing the 4T1 breast cancer mice model showed that the alternated cooling and heating treatment not only induced severely vascular injury, but also the anti-tumor immune response, which is better than any of the existing thermal therapy. Method： Investigate the cellular injury response of breast cancer cell line MDA-MB-231 induced by different physical conditions, and establish the VX2 breast tumor model of rabbit, which was preliminary verified by combination of pathological analysis and imaging methods. Result： We found that alternated cooling and heating treatment resulted in more severely cellular injury; moreover, a good recovery of VX2 Rabbit treated by altemated cooling and heating was proved without metastasis and recurrence appeared in three month. Conclusion： Alternated Cooling and Heating Treatment is art effective method for tumor therapy.

关 键 词：冷热交替 细胞损伤 新西兰大白兔乳腺肿瘤 

分 类 号：Q813.1[生物学—生物工程]