异氟烷对成龄和老龄大鼠海马蛋白质组延迟相的差异性影响  

Delayed effect of isoflurane on hippocampal proteome after anesthesia in adult and aged rats

作　　者：段开明[1] 欧阳文[1] 陈满红[1] 夏月峰[1] 汪赛赢[1] 

机构地区：[1]中南大学湘雅三医院麻醉科,长沙410013

出　　处：《中南大学学报（医学版）》2009年第7期589-594,共6页Journal of Central South University ：Medical Science

基　　金：国家自然科学基金(3087136);湖南省自然科学基金(07JJ5039)~~

摘　　要：目的:应用双向凝胶蛋白电泳和质谱分析技术筛选和鉴定异氟烷麻醉对老龄和成龄大鼠海马蛋白质组的延迟相影响。方法:将10只8月龄SD大鼠随机分为Cadult组和Iadult组(n=5),10只22月龄SD大鼠随机分为Caged组和Iaged组(n=5)。Cadult和Caged组吸入含40%氧气的空气和氧气混合气体2h;Iadult和Iaged组以3%异氟烷和40%氧气吸入诱导,随后以1.2%异氟烷和40%氧气维持全身麻醉2h。于吸氧对照或麻醉结束后24h取海马组织行双向凝胶电泳和质谱分析以鉴定差异蛋白点。结果:Iadult组和Iaged组大鼠麻醉过程中生命体征平稳。获得了较满意的双向凝胶电泳胶图,图像分析结果显示,Cadult和Iadult组合成胶蛋白质点分别为878±34和864±49;Caged组和Iaged组合成胶蛋白质点分别为834±47和819±24。Iadult组和Cadult组差异蛋白质点共有12(4/8)个;Iaged组和Caged组差异蛋白质点共有11个(3/8)。质谱分析显示,这些蛋白质分别属于代谢相关蛋白质、抗氧化蛋白质以及调节蛋白质。结论:异氟烷麻醉可以引起大鼠海马蛋白质组延迟相变化,并且这些变化具有年龄差异性。Objective To investigate the delayed alteration of hippocampus proteome after anesthesia with isoflurane in adult and aged rats. Methods Ten 8-month-old SD rats were randomly divided into group Cadult and group Iadult （5 in each group ） , and another ten 22-month-old SD rats were randomly divided into group Caged and group Iaged （5 in each group ）. The rats in group Iadult and group laged received 2 h anesthesia with 1.2 % isoflurane. The rats in group Cadult and group Caged inhaled 40% oxygen for contrast. The hippocampal proteome of each rat was measured by 2-dimensional gel electrophoresis and mass spectrometry. Results The vital signs of the rats in group Iadult and group Iaged were stable. There were 878 ± 34 protein spots in group Cadult, 864 ±49 protein spots in group Iadult, 834 ±47 in group Caged, and 819 ± 24 in group Iaged. There were 12 （4/8）different protein spots between group Iaduh and group Caduh. There were 1 1 （3/8） different protein spots between group Iaged and group Caged. All of the protein spots were identified by MALDI-TOF-MS. Most of the different proteins were related to metabolism, anti-oxidation, and signal conditioning of synapse. Conclusion Isoflurane may cause the alteration of hippocampal proteome in rats, which is age-related.

关 键 词：异氟烷 海马 延迟相 蛋白质组 老龄 大鼠 

分 类 号：R96[医药卫生—药理学]