乙型肝炎病毒前S1蛋白抗原检测的临床意义  

Clinical significance of detection of HBV PreS1 protein antigen

作　　者：王槐堂 魏中南[2] 

机构地区：[1]湖北省咸宁市结核病防治所检验科,437100 [2]湖北省妇幼保健院检验科,武汉430070

出　　处：《中国医药生物技术》2009年第5期364-366,共3页Chinese Medicinal Biotechnology

摘　　要：目的通过分析HBV前S1(PreS1)蛋白抗原和血清标志物之间的关系,探讨PreS1蛋白抗原检测在临床上的应用价值。方法收集382例HBV患者血清样本,采用ELISA法检测HBVPreS1蛋白抗原和血清标志物,采用荧光定量PCR法检测HBV-DNA,并比较所有检测结果。结果382例HBV患者血清样本PreS1蛋白抗原和HBV-DNA的检出率分别为33.8%和40.8%;其中HBeAg阳性的144例中,PreS1抗原阳性为112例,HBV-DNA阳性为134例,阳性率分别为77.8%和93.1%;HBeAg阴性的102例中,PreS1抗原阳性为17例,HBV-DNA阳性为22例,阳性率分别为7.1%和9.2%。HBV-DNA检出率稍高于PreS1抗原的检出率,但两者间检出率差异均无统计学意义(P>0.05)。结论HBVPreS1蛋白可反映HBV复制的情况,与HBV-DNA结果有较好的一致性,可作为临床上HBV感染诊断或监测的指标。Objective Through comparing the indicative role of HBV PreS 1 antigen with that of HBV-DNA when both used to diagnose HBV samples, which were diagnosed by serum markers, to explore the clinical application value of HBV PreS1 antigen in diagnosing HBV. Methods With respect to 382 cases, to detect serum HBV markers, PreS1 antigen and HBV-DNA by using enzyme-linked immunosorbent assay （ELISA） and fluorescent quantity polymerase chain reaction （FQ-PCR）, respectively, then to test statistic significance of their difference. Results For these 382 HBV cases, PreS1 antigen and HBV-DNA indicative rates were 33.8% and 40.8%, respectively; for 144 cases with HBeAg（＋）, ll2-case were PreS1 antigen positive, 134-case HBV-DNA positive, and the indicative rates were 77.8% and 93.1%, respectively; for 102 cases with HBeAg（-）, 17-case were PreS1 antigen positive, 22-case HBV-DNA were positive, the indicative rates were 7.1% and 9.2%, respectively. HBV-DNA indicative capacity was slightly higher than that of PreS 1 antigen, but the differences were not statistically significant （P 〉 0.05）. Conclusion HBV PreS 1 protein can reflect the status of HBV replication, basically are consistent with HBV-DNA in testing results, and can be used in clinically diagnosing and monitoring HBV.

关 键 词：肝炎病毒 乙型 肝炎e抗原 乙型 前S1蛋白 

分 类 号：R512.62[医药卫生—内科学] R446.62[医药卫生—临床医学]