UVA1对人工皮肤成纤维细胞光损伤的影响  

The photo-damage effect of UVA1 irradiation to dermal fibroblasts in the artificial skin

作　　者：孙素姣[1] 苏顺琴[1] 徐继鹏[1] 姚露[1] 罗雯[1] 何黎[1] 

机构地区：[1]昆明医学院第一附属医院皮肤科,云南昆明650032

出　　处：《皮肤病与性病》2009年第3期1-3,共3页Dermatology and Venereology

基　　金：云南省科技厅应用基础研究;项目编号:2007C0011Z

摘　　要：目的探讨不同UVA1照射剂量对人工皮肤真皮细胞形态及细胞活性的影响,为建立人工皮肤光损伤模型提供依据。方法以不同剂量UVA1(0J/cm2-80J/cm2)照射人工皮肤,通过HE染色,从形态学上观察经UVA1照射后真皮成纤维细胞数量的变化,通过MTT法检测真皮成纤维细胞的活性。结果HE染色示真皮成纤维细胞的数量随UVA1剂量的增大而减少,于真皮浅层较为明显;MTT法示与0J/cm2组相比,20J/cm2和30J/cm2UVA1照射人工皮肤时成纤维细胞活性未受到明显的抑制(P>0.05),UVA1剂量大于40J/cm2时细胞活性下降明显(P<0.05,P<0.001),呈剂量依赖性。结论UVA1照射剂量大于40J/cm2是造成人工皮肤真皮成纤维细胞光损伤的始剂量。Objective The different doses of UVA1 were screened to investigate their influences to the morphology and cell activity of the dermal of artificial skin in order to provide a theoretical basis of dermal of artificial skin photo - damage model. Methods After different doses of UVAI exposure（0J/cm^2 - 80J/cm^2 ）, the number of changes of dermal fibroblasts was observed from the morphology by histology staining, and the dermal fibroblast activity was measured by the MTT assay. Results Histology staining showed that major alterations of the number of dermis fibroblasts were observed within the superficial part of dermal equivalent, and inversely with the increase of the UVA1 dose. MTT assay showed that there was no obvious depression of fibroblast activity in group 20J/cm^2 and group 30J/cm^2 （ P 〉 0.05） after UVA1 exposure. Fibroblast activity was decreased obviously （ P 〈 0.05,P 〈 0. 001 ） when the dose was more than 40 J/cm^2 , and this change appeared dose - dependent. Conclusion The dose of UVA1 irradiation which was higher than 40J/cm^2 was orginal dose leading to the dermal fibroblast photo - damage of artificial skin.

关 键 词：长波宽谱紫外线(UVA1) 人工皮肤 光损伤 

分 类 号：R454.2[医药卫生—治疗学] R322.991[医药卫生—临床医学]