蛇毒抗补体蛋白atrase B抑制补体活化引起的血小板聚集  

Inhibitory effect of anticomplementary protein atrase B from cobra venom on platelet aggregation induced by activated complement

作　　者：王彩娥[1,2] 孙黔云[1] 李敏[3] 石京山[2] 

机构地区：[1]贵州省中国科学院天然产物化学重点实验室,贵州贵阳550002 [2]遵义医学院,贵州遵义563000 [3]贵州省人民医院,贵州贵阳550002

出　　处：《中国药理学通报》2009年第9期1205-1209,共5页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助项目(No30560035);国家重点基础研究发展计划(973计划)资助项目(No2009CB526512、2007CB516813);贵州省科技攻关资助项目(No黔科合工计字2002-1075号);贵州省优秀青年科技人才资助项目(No黔科合人字2005-0510号);贵州省优秀科技教育人才省长专项资金项目

摘　　要：目的观察眼镜蛇毒抗补体蛋白atrase B抑制补体活化引起的血小板聚集。方法采用眼镜蛇毒因子激活补体诱导血小板聚集,观察atrase B对补体活化引起人凝胶过滤血小板聚集的影响,并采用流式细胞仪测定血小板膜P-选择素和GPⅡb/Ⅲa表达情况。结果Atrase B能抑制补体激活引起的血小板聚集和P-选择素、GPⅡb/Ⅲa在血小板膜上的表达。结论眼镜蛇毒抗补体蛋白atrase B能有效抑制补体激活引起的血小板活化、聚集。Aim To investigate the inhibitory effect of atrase B on human platelet aggregation induced by activated complement. Methods By employing CVF to activate complement, the effect of atrase B on gel filtered platelet aggregation induced by activated complement was measured by turbidimetry and the expression of P-selectin and GP Ⅱb/Ⅲ a on platelet membrane were detected by flow cytometry. Results Atrase B inhibited platelet aggregation and the expression of P-selectin and GP Ⅱb/Ⅲ a on by activated complement. platelet membrane induced Conclusion Anticomplementary protein atrase B from Naja atra venom can significantly inhibit platelet activation and aggregation induced by activated complement.

关 键 词：抗补体蛋白 补体活化 血小板活化 血小板聚集 眼镜蛇毒因子 蛇毒 P-选择素 糖蛋白GPⅡb/Ⅲa 

分 类 号：R331.124[医药卫生—人体生理学] R392.11[医药卫生—基础医学]