Inhibitory effects and mechanisms of high molecular-weight phlorotannins from Sargassum thunbergii on ADP-induced platelet aggregation  

作　　者：魏玉西 王长云 李敬 郭奇 齐宏涛 

机构地区：[1]Biological Department,Qingdao University [2]Key Laboratory of Marine Drugs,Ministry of Education,School of Medicine and Pharmacy,Ocean University of China [3]Institute of Oceanology,Chinese Academy of Sciences

出　　处：《Chinese Journal of Oceanology and Limnology》2009年第3期558-563,共6页中国海洋湖沼学报（英文版）

基　　金：Supported by the National Natural Science Foundation of China (No 30572314);the Basic Research Program of Science and Technology,Ministry of Science and Technology of China (2007FY210500);the Program of Chinese Offshore Investigation and Assessment,State Oceanic Administration of China (Nos 908-01-ST12 and 908-02-05-04);the Science and Technology Planning Project of Qingdao (No 06-2212-JCH)

摘　　要：We evaluated the effects of high molecular-weight phlorotannins from Sargassum thunbergii(STP) on ADP-induced platelet aggregation and arachidonic acid(AA) metabolism in New Zealand white rabbits and Wistar rats.The inhibition of STP on platelet aggregation was investigated using a turbidimetric method,and the levels of the terminal products of AA metabolism were measured using the corresponding kits for maleic dialdehyde(MDA),thromboxane B2(TXB2) and 6-keto-prostaglandin F1α(6-keto-PGF1α) by colorimetry and radioimmunoassay,as appropriate.We found that STP could inhibit ADP-induced platelet aggregation,and the inhibitory ratio was 91.50% at the STP concentration of 4.0 mg/mL.Furthermore,STP markedly affected AA metabolism by decreasing the synthesis of MDA(P<0.01) and increasing the synthesis of 6-keto-PGF1α,thus changing the plasma TXB2/6-keto-PGF1α balance when the platelets were activated(P<0.01).Therefore,STP altered AA metabolism and these findings partly revealed the molecular mechanism by which STP inhibits ADP-induced platelet aggregation.We evaluated the effects of high molecular-weight phlorotannins from Sargassum thunbergii （STP） on ADP-induced platelet aggregation and arachidonic acid （AA） metabolism in New Zealand white rabbits and Wistar rats. The inhibition of STP on platelet aggregation was investigated using a turbidimetric method, and the levels of the terminal products of AA metabolism were measured using the corresponding kits for maleic dialdehyde （MDA）, thromboxane B2 （TXB2） and 6-keto-prostaglandin F1α （6-keto-PGF1α） by colorimetry and radioimmunoassay, as appropriate. We found that STP could inhibit ADP-induced platelet aggregation, and the inhibitory ratio was 91.50% at the STP concentration of 4.0 mg/mL. Furthermore, STP markedly affected AA metabolism by decreasing the synthesis of MDA （P〈0.01） and increasing the synthesis of 6-keto-PGF1α, thus changing the plasma TXB2/6-keto-PGF1α balance when the platelets were activated （P〈0.01）. Therefore, STP altered AA metabolism and these findings partly revealed the molecular mechanism by which STP inhibits ADP-induced platelet aggregation.

关 键 词：high molecular-weight phlorotannins Sargassum thunbergii Kuntze platelet aggregation arachidonic acid metabolism 

分 类 号：R96[医药卫生—药理学]