RAS相关结构家族基因1克隆及序列分析  

Gene clone and sequence analysis of RASSF1B

作　　者：王崇高[1] 陈吉祥[2] 胡升庠[1] 赵新潮[1] 卢明[1] 

机构地区：[1]南京市第一医院集团南京市浦口区中心医院普外科,江苏南京211800 [2]江苏大学附属医院普外科,江苏镇江212001

出　　处：《医学研究生学报》2009年第9期944-946,950,共4页Journal of Medical Postgraduates

基　　金：镇江市科技局基金资助项目(批准号:SH2005046)

摘　　要：目的:RASSF1家族与癌变的发生密切相关。为探究RASSF1家族与癌变发生生理机制的相关性,分析该家族中RASSF1B的生物学功能。方法:应用RT-PCR的方法结合生物信息学,初步探索基因RASSF1B的生物学功能。结果:该基因在所测试的几种肿瘤细胞中均有表达,但表达量有差异。通过生物信息学得知,该基因含有1个Ras结合区域和1个SARAH区域。结论:RASSF1B基因的编码产物功能结构域在肿瘤的发生过程中起作用。Objective： The RASSF1 gene family are remarkably involved in the development of cancer. This study aimed to investigate the relationship between cancer development and the RASSFI gene family and the biological function of the RASSF1B gene. Methods： The biological function of the RASSF1 B gene was studied by RT-PCR and biological informaties analysis. Results： The RASSF1B gene expressed in the tested tumor cells in different degrees. Biological informatics analysis showed that the RASSF1 B gene contained a Ras protein binding domain and an SARAH region. Conclusion ： The product of the RASSF1 B gene plays a role in the tumor development.

关 键 词：克隆 癌变 RAS相关结构家族基因1 

分 类 号：Q785[生物学—分子生物学]