狂犬病毒Flury株糖蛋白cDNA克隆与序列分析  

Cloning and sequencing of Glycoprotein cDNA of Rabies virus Flury strain

作　　者：唐婕[1] 陈铁桥[2] 李六金[3] 

机构地区：[1]陕西省动物研究所,陕西西安710032 [2]湖南农业大学动物医学院 [3]第四军医大学实验动物中心

出　　处：《中兽医医药杂志》2009年第5期5-7,共3页Journal of Traditional Chinese Veterinary Medicine

摘　　要：根据狂犬病毒糖蛋白核苷酸序列,利用Oliga软件设计两对特异性引物,通过反转录-聚合酶链式反应(RT-PCR)扩增了狂犬病毒Flury-lep糖蛋白的全长cDNA,将其插入克隆载体pMD-18T并测序。测序结果及同源性分析表明,糖蛋白cDNA长1574bp,编码524个氨基酸。狂犬病毒株Flury-lep的RGP基因序列与GenBank公布的狂犬病毒株RGP基因片段核苷酸序列的同源性为82.3%～96.3%,其编码产物的氨基酸序列同源性为88.4%～94.3%。Aeeording to GenBank of the rabies virus glycoprotein gene,two pairs of speeifie primere were designed using Oliga software.Amplified the full-length cDNA fragmenl of GP gene of Flury strain by reverse transeription-polymerase chain reaction（RT-PCR）and cloned nto pMD-18T vector for sequeneing.Sequenee and homology analyisis showed that the cDNA of RGP encoding 524 amino acids was consisted of 1574 nucleic acids.The homologies of nucleic acid and amino acid sequences to those reported were82.3%-96.3% and 88.4%-94.3%.respectively.

关 键 词：狂犬病毒 糖蛋白 克隆 序列分析 

分 类 号：S852.655[农业科学—基础兽医学]