草鱼HSP70基因cDNA部分序列克隆及其组织表达差异  

Cloning of HSP70 partial cDNA sequence and its tissue differential expression in grass carp(Ctenopharyngodon idella)

作　　者：林亚秋[1] 郑玉才[1] 吉红[2] 

机构地区：[1]西南民族大学生命科学与技术学院,成都610041 [2]西北农林科技大学动物科技学院水产系,陕西杨凌712100

出　　处：《淡水渔业》2009年第4期67-71,共5页Freshwater Fisheries

基　　金：国家自然科学基金资助资项目(30771667)资助

摘　　要：根据鲤热休克蛋白70(Heat shock protein,HSP70)序列(AY120894)设计并合成一对引物,以草鱼(Cteno-pharyngodon idella)肝胰脏组织总RNA为模板,RT-PCR扩增获得草鱼HSP70基因cDNA部分序列,并进行了组织表达差异性研究。结果显示:所获为序列为480 bp,获得GeneBank登陆号为FJ483832。序列测序结果显示,HSP70扩增序列与鲤、斑马鱼、鲋的同源性为:93%、91%、93%。另外,所获序列HSP70在草鱼脂肪、肌肉、肠、脑、粘液、性腺、鳔、肝胰脏、心脏、脾脏、鳃、鳍12个组织的表达存在差异,HSP70在草鱼这12个组织中均检测到表达,其中在鳍中表达最高,极显著高于其他组织(P<0.01);在鳔中表达次之,且与脑、心脏、性腺中表达差异不显著;在粘液中表达最低。A pair of primers was designed according to carp HSP70 sequence（AY120894）.Total RNA was extracted from the hepatopancreas of grass carp.480 bp of grass carp HSP70 gene was obtained by RT-PCR and given number FJ483832 by GeneBank.It was found that HSP70 gene of grass carp had a homolog of 93%,91% and 93% with carp,zebrafish and Carassius auratus,respectively,using DNAman software.Moreover,we examined the mRNA expression of HSP70 in various tissues of grass carp,such as adipose tissue,muscle,hepatopancreas,spleen,gonad,gill,fin,swim bladder,brain,mucilage,heart and intestine,by semi-quantitative（SQ RT-PCR） method.The data showed that HSP70 gene was all detected but different in tested tissues.The highest was found in fin,which was significantly higher than other tissues（P〈0.01）,the next highest was swim bladder,which had no obvious difference with brain,heart and gonad,and the lowest was mucilage.These results suggest that HSP70 may be used as a stress-involved index in fish culture.

关 键 词：热休克蛋白(HSP70) 克隆 组织差异 草鱼(Ctenopharyngodon idella) 

分 类 号：S917[农业科学—水产科学] Q786[生物学—分子生物学]