创伤患者外周血淋巴细胞自噬变化及其与糖代谢的关系  

A study on the change of autophagy in peripheral blood lymphocytes following trauma

作　　者：余剑[1] 张连阳[1] 韩荣鹏[1] 梁华平[1] 

机构地区：[1]第三军医大学大坪医院野战外科研究所全军战创伤中心,重庆400042

出　　处：《中华实验外科杂志》2009年第10期1295-1297,共3页Chinese Journal of Experimental Surgery

基　　金：国家重点基础发展规划“973”计划资助项目（2005CB522602）;国家自然科学基金资助项目（30772253）

摘　　要：目的观察创伤患者外周血淋巴细胞的自噬变化规律以及与创伤后血糖变化的关系。方法根据损伤严重度评分（ISS）或简明损伤定级标准（AIS）将创伤患者分为轻伤组、重伤组、严重伤组，同时设立健康对照组，分别于创伤后1、3、5、10d采集外周血，采用密度梯度离心结合贴壁分离法分离创伤患者外周血淋巴细胞，应用激光共聚焦显微镜和透射电镜观察细胞自噬现象；将分离得到的淋巴细胞进行MDC染色并裂解，应用荧光分光光度计（激发光波长380nm、发射光波长525nm）测定荧光强度进行定量分析；于创伤后1、3、5d检测患者血糖水平，分析创伤后自噬与血糖的相关性。结果与健康对照组比较，创伤患者外周血淋巴细胞自噬水平明显升高，有显著变化（P〈0．01）。结论创伤后外周血淋巴细胞自噬水平与创伤严重程度、创伤后血糖成正相关。Objective To investigate the change rule of autophagy in peripheral blood lymphocytes in trauma patients and its relationship with the change of blood sugar after trauma. Methods According to ISS or AIS, all the patients were divided into three groups：mild, moderate, and severe trauma groups. The control group was established. The peripheral blood of three trauma groups was collected at the day 1,3,5 and 10 after trauma, and lymphocytes were obtained by density gradient centrifugation combined with adherence separation. The autophagy phenomenon was observed by using laser confocal microscopy and transmission electron microscope. The lymphoeytes were stained with MDC, and lysed for quantitative detection of the fluorescent intensity by fluorospeetrophotometer （ wavelength of excitation light was 380 nm,and that of emission light was 525 nm）. The blood sugar levels were determined at the 1st,3rd, and 5th day respectively after trauma, and the correlation between autophagy and blood sugar levels was analyzed. Results Compared with control group, the level of autophagy in lymphocytes in trauma patients was significantly increased in trauma groups （ P 〈 0.01 ）. Conclusion The level of autophagy in lymphocytes after trauma had a positive correlation with the trauma degree and the blood sugar.

关 键 词：创伤 淋巴细胞 自噬 糖代谢 

分 类 号：R64[医药卫生—外科学]