鳜肌球蛋白轻链2基因cDNA的克隆及其发育表达分析  

cDNA Cloning and Ontogenetic Expression Analysis of the Myosin Light Chain 2 Gene in Siniperca Chuasti

作　　者：周瑞雪[1,2] 蒙涛[1,2] 褚武英[2] 成嘉 李志英[2] 宾石玉[1] 张建社[2] 

机构地区：[1]广西师范大学生命科学学院,中国桂林541004 [2]长沙学院生物工程与环境科学系,中国长沙410003

出　　处：《湖南师范大学自然科学学报》2009年第3期78-83,共6页Journal of Natural Science of Hunan Normal University

基　　金：国家自然科学基金资助项目(30771644);湖南省自然科学基金资助项目(K082014-13);湖南省教育厅资助项目(08C133)

摘　　要：通过构建肌肉组织cDNA文库分离到鳜肌球蛋白轻链2基因(MLC2),基因登录号为FJ428249.MLC2基因cDNA序列全长1206bp,编码区长度为579bp.MLC2基因开放阅读框编码170个氨基酸,具有EF-手相家族蛋白全部4个EF-手相结构.与已报道的其他动物MLC2相比较,所推导氨基酸序列同源性在66.3%～97.6%,其中与Ca2+结合区域非常保守,7种鱼氨基酸序列同源性为100%.采用实时荧光定量PCR方法对鳜MLC2发育表达分析表明,MLC2在原肠期开始有低量表达,与原肠期相比,尾芽期、肌肉效应期和仔鱼阶段MLC2表达量随发育阶段渐进而升高.The myosin light chain 2（MLC2） gene was isolated from the muscle tissue cDNA library of Siniperca chuasti. The length of the MLC2 cDNA is 1 206 bp, and the open reading frame is 579 bp. The MLC2 encodes 170 amino acids, which includes four EF-hand structures. Compared with the MLC2 of other animals reported, the homology of the deduced amino acids sequences was between 66.3% -97.6%. The primary structures of the Ca^2＋ - binding domain were well conserved among the MLC2s of seven other fish species. The ontogenetic expression analysis by real-time PCR showed that the MLC2 mRNA was first detected in gastrula stage and its expression gradually increased from the tail bud stage to larval stage.

关 键 词：鳜 肌球蛋白轻链2 CDNA克隆 发育表达 

分 类 号：Q785[生物学—分子生物学]