DHPLC法检测TCF7L2基因多态性与2型糖尿病的相关性  

Study on Polymorphisms of TCF7L2 Gene Association with Type 2 Diabetes Based on DHPLC Detection

作　　者：樊向维[1] 刘德敏[1] 孙颖[1] 张捷[1] 史晓文[1] 

机构地区：[1]天津医科大学代谢病医院、卫生部及天津市激素与发育重点实验室,300070

出　　处：《天津医药》2009年第10期835-838,共4页Tianjin Medical Journal

摘　　要：目的:探讨天津地区汉族人群TCF7L2基因rs12255372位点多态性与2型糖尿病(T2DM)发生的相关性。方法:随机抽取352例T2DM患者及176例健康对照者,提取基因组DNA,PCR扩增目的片段后,应用变性高效液相色谱技术(DHPLC)检测该位点基因型,并抽取不同峰型进行DNA测序。分析不同基因型频率的组间差异,Logistic回归分析相关危险因素。结果:TCF7L2基因rs12255372位点的G/T杂合基因型在T2DM组和对照组中的频率分别为18.5%和2.8%,差异有统计学意义(P<0.01)。G/T基因型、糖化血红蛋白、尿素氮、收缩压为2型糖尿病的独立危险因素,高密度脂蛋白为保护因素。T2DM不同并发症患者G/T基因型频率差异无统计学意义(P>0.05)。结论:TCF7L2基因的rs12255372位点G/T基因型携带者发生T2DM危险性增大,该位点可能与天津地区汉族人群T2DM的发病有关,是2型糖尿病发生的危险因素之一。Objective： To detect the relationship between variant of rs1255372 in transcription factor 7-Like 2 （TCF7L2） gene and the type 2 diabetes mellitus （T2DM） in Tianjin Han populations. Methods： Three hundred and fifty-two T2DM patients and 176 healthy controls were randomly selected to extract the genome DNA. Denaturing high performance liquid chromatography was used to detect the PCR amplified fragment. The different peaks were chosen for sequencing. The differences of different genotype frequency between the groups were analysed. The Logistic regression was used to evaluate the risk factors of T2DM. Results： The G/T genotype frequencies of rs12255372 were 18.5% and 2.8% in T2DM group and healthy control group respectively （P 〈 0.05）. The GfF genotype, glycosylated hemoglobin, urea nitrogen and the systolic blood pressure were the independent risk factors of T2DM, and the high density lipoprotein was the protective factor. There was no significant difference of the Gfr genotype frequency in T2DM patients with different complications. Conclusion： This study indicates that G/T genotype in the TCF7L2 gene significantly contributes to T2DM susceptibility in Tianjin Han populations. The G/T genotype is one of the risk factors of the T2DM.

关 键 词：糖尿病 2型 多态现象 遗传 色谱法 液相 LOGISTIC模型 汉族 天津 

分 类 号：R587.1[医药卫生—内分泌] R541.4[医药卫生—内科学]