氯胺酮对大鼠肾脏低温延时保存影响的形态学观察  

Effect of ketamine dissolved in multi-organ preservation solution on morphology of cold-stored rat kidneys

作　　者：范颖晖[1] 高轶[2] 王新华[1] 

机构地区：[1]同济大学附属东方医院疼痛科,上海200120 [2]第二军医大学长征医院泌尿外科,上海200003

出　　处：《第二军医大学学报》2009年第9期1018-1021,共4页Academic Journal of Second Military Medical University

摘　　要：目的:观察氯胺酮对大鼠肾脏低温保存期间肾皮质形态学的影响。方法:雄性SD大鼠分别用0～4℃单纯高渗枸椽酸盐嘌呤溶液(HC-A)保存液、加入氯胺酮10-5μmol/L的HC-A保存液50～100ml原位逆行灌注肾脏,离体低温保存24、48、72h,取材在光镜和电镜下作形态学观察。结果:低温保存72h内氯胺酮组肾脏光镜和电镜下组织形态学有明显改变,细胞和细胞器变性坏死,肾间质高度水肿;单纯HC-A保存液组上述改变较轻。结论:在器官保存液中加入氯胺酮,不利于肾脏低温保存。Objective：To observe the morphological changes of cold-stored rat kidneys flushed with multi-organ preservation solution containing ketamine.Methods：The kidneys of male SD rats were retrogradely perfused with 0-4℃ HC-A（50-100 ml） only or together with ketamine（10-5μmol/L）,and were then isolated,cold stored for 24,48,and 72 h.The morphological changes of the kidneys in both groups were observed under light microscope and transmission electron microscope.Results：Light microscopy and transmission electron microscope. Results： Light microscopy and transmission electron microscopy showed that there were evident morphological changes in the ketamine group after 72 h, with necrotic cells and organelles, and severe renal interstitial edema. The above changes in the HC-2 group were slighter. Conclusion： Ketamine in the organ preservation solution is harmful for the cold storage of rat kidneys.

关 键 词：氯胺酮 肾 低温保存 肾移植 

分 类 号：R699.2[医药卫生—泌尿科学]