多药耐药基因1和多药耐药相关蛋白基因1在腺样囊性癌细胞系ACC-2及其耐药细胞系ACC-2/BLM中表达的研究  

Multidrug resistance gene1 and multidrug resistance-associated protein gene1 expression in human salivary gland adenoid cystic carcinoma cell line ACC-2 and multidrug resistant cell line ACC-2/BLM

作　　者：秦楠[1] 缪叶[1] 刘峰[1] 李焰[1] 吴军正[1] 

机构地区：[1]第四军医大学口腔医学院口腔生物学教研室,西安710032

出　　处：《口腔医学》2009年第9期449-452,共4页Stomatology

基　　金：国家自然科学基金(30672343)

摘　　要：目的检测多药耐药基因1(MDR1)和多药耐药相关蛋白基因1(MRP1)在腺样囊性癌细胞系ACC-2及其耐药细胞系ACC-2/BLM中的表达。方法应用RT-PCR和免疫细胞化学技术分别检测MDR1和MRP1的mRNA表达以及相对应的P-gp和MRP1蛋白在两组细胞系中的表达。结果RT-PCR检测结果显示ACC-2以及ACC-2/BLM细胞均不表达MDR1mRNA,MRP1mRNA在ACC-2/BLM细胞中的表达明显强于ACC-2细胞。免疫细胞化学结果显示,2组细胞均不表达P-gp,在ACC-2/BLM细胞中MRP1蛋白表达强于ACC-2细胞。结论MRP1基因及其编码的MRP1蛋白在ACC-2/BLM细胞中高表达,可能与ACC-2/BLM细胞系产生多药耐药有关。Objective To detect the expressions of multidrug resistance genel （ MDR1 ） and multidmg resistance - associated protein genel （ MRP1 ） in human salivary gland adenoid cystic carcinoma cell line ACC-2 and muhidrug resistant cell line ACC-2/ BLM. Methods RT-PCR was employed to test the expressions of MDR1 and MRP1 mRNA in ACC-2/BLM and ACC -2/BLM cell lines. Immunoeytochemistry method was used to examine the expression of muhidrug resistance protein（P-gp） and muhidrug resistanceassociated proteinl （ MRP1 ） in the cell lines. Results The expressions of MRPI mRNA and MRP1 in ACC-2/BLM cells were apparently higher than those in ACC-2 cells. But MDRI mRNA and P-gp were not observed in ACC -2/BLM and ACC-2 cells. Conclusion The expressions of MRP1 mRNA and MRP1 may be related to the multidrug resisitanee of ACC-2/BLM cells.

关 键 词：腺样囊性癌 多药耐药性 多药耐药蛋白 多药耐药相关蛋白 

分 类 号：R349.6[医药卫生—基础医学]