二肽基肽酶Ⅳ在2型糖尿病大鼠牙龈表达的免疫组化研究  

Immunohistochemistry study on DPP-Ⅳ in rats treated with high fat diet and streptozotocin

作　　者：毛钊[1] 杨俭[2] 徐辉[1] 

机构地区：[1]南京军区南京总医院口腔科,南京210002 [2]南京医科大学基础医学院药理系,南京210029

出　　处：《口腔医学》2009年第9期457-460,487,共5页Stomatology

基　　金：国家自然科学基金面上项目(30772616);中国人民解放军南京军区十五医药卫生基金(02MB003)

摘　　要：目的观察2型糖尿病时大鼠牙龈组织中DPP-Ⅳ分布和表达情况。方法将实验动物分为高脂糖饮食组、2型糖尿病组和对照组,以高脂糖饮食加链脲霉素腹膜内注射制备2型糖尿病动物模型,根据葡萄糖耐量试验异常确定2型糖尿病动物模型成功。采用免疫组化染色法观察二肽基肽酶Ⅳ(DPP-Ⅳ/CD26)在肠、肾和牙龈中的分布和表达。结果DPP-Ⅳ主要定位于肠上皮、肾小管细胞和牙龈黏膜上皮细胞。在肠和肾组织无论高脂糖饮食组(HF)还是2型糖尿病模型组(DM)DPP-Ⅳ表达均强于对照组。在牙龈组织,DM组大鼠强阳性表达,HF组大鼠弱阳性表达,对照组大鼠阴性表达。结论在2型糖尿病时,DPP-Ⅳ/CD26在大鼠牙龈组织中出现高表达。Objective To observe expression of DPP-Ⅳ in intestine, kidney and gingival of the rats with Type Ⅱ diabetes. Methods Three groups of rats （ control ,high fat diet （HF） and HF plus STZ for Type Ⅱ diabetes mellitus （DM） ） were monitored with plasma glucose and insulin for 12 weeks. Type Ⅱ diabetic rats were confirmed by the glucose/insulin tolerance test. The expressions of DPP-Ⅳ in intestine, kidney and gingival were examined using immunohistochemistry. Results Compared with rats on the normal diet, the expression of DPP-Ⅳ was significantly increased in the intestine, kidney and gingival in rats with Type Ⅱ diabetes. The rats with high fat diet also moderately upregulated DPP-Ⅳ expression in intestine ,kidney and gingival. Conclusion These findings suggested that the overexpression of DPP-Ⅳ on gingival in Type Ⅱ diabetes could affect health of periodontal tissues .

关 键 词：DPP-Ⅳ/CD26 2型糖尿病 高脂糖饮食 免疫组化 牙龈 

分 类 号：R349.5[医药卫生—基础医学]