Study on Haploid Inducing and Its Meiotic Abnormality in Maize  

作　　者：TANG Qi-lin, FENG Yun-chao, HAN Xue-li, ZHENG Ming-min and RONG Ting-zhao Maize Research Institute, Sichuan Agricultural University, Ya’an 625014, P.R.China 

出　　处：《Agricultural Sciences in China》2009年第10期1159-1165,共7页中国农业科学（英文版）

基　　金：supported by the National 973 Program of China (2007CB108900);the National Natural Science Foundation of China (30671308);SichuanYouth Science and Technology Fund, China (2007Q14-032);Ministry of Education Innovation Team Development Plan, China (IRT0453);Sichuan Forage Grass Breeding Project, China

摘　　要：The haploid-inducing line Stock 6 was used to produce haploid maize and expected to obtain maize haploid plants successfully. The detailed meiotic studies on selected haploid maize （n = x = 10） were conducted. Cytogenetic analysis revealed a high frequency of meiotic abnormality occurred in both meiosis Ⅰ and meiosis Ⅱ. During the prophase Ⅰ, univalents were common configurations, and there were bivalents or trivalents in some pollen mother cells, however, a few cells containing five bivalents were also observed. After prophase Ⅰ, chromosomes did not congregate in a single metaphase plate but they were scattered in the cytoplasm. At anaphase Ⅰ, the chromosome distribution was highly irregular with almost all possible combinations. In some cells, chromosomes were grouped into the three or four masses and several spindles appeared. At the tetrad stage of meiosis Ⅱ, eytokinesis splitting abnormality occurred, and a variety of diad, triad, tetrad, pentad, hexad, as well as decury microspores were easily observed. As a consequence of abnormalities of the two meiotic stages, various microspores and the pollen were almost completely sterile. grains with different size were formed, and its pollen grainsThe haploid-inducing line Stock 6 was used to produce haploid maize and expected to obtain maize haploid plants successfully. The detailed meiotic studies on selected haploid maize （n = x = 10） were conducted. Cytogenetic analysis revealed a high frequency of meiotic abnormality occurred in both meiosis Ⅰ and meiosis Ⅱ. During the prophase Ⅰ, univalents were common configurations, and there were bivalents or trivalents in some pollen mother cells, however, a few cells containing five bivalents were also observed. After prophase Ⅰ, chromosomes did not congregate in a single metaphase plate but they were scattered in the cytoplasm. At anaphase Ⅰ, the chromosome distribution was highly irregular with almost all possible combinations. In some cells, chromosomes were grouped into the three or four masses and several spindles appeared. At the tetrad stage of meiosis Ⅱ, eytokinesis splitting abnormality occurred, and a variety of diad, triad, tetrad, pentad, hexad, as well as decury microspores were easily observed. As a consequence of abnormalities of the two meiotic stages, various microspores and the pollen were almost completely sterile. grains with different size were formed, and its pollen grains

关 键 词：Zea mays L haploid maize MEIOSIS CHROMOSOME Stock 6 

分 类 号：S513[农业科学—作物学]