苹果MdFT基因对番茄的遗传转化  

Genetic Transformation of Apple MdFT Gene into Tomato

作　　者：李伟明[1,2] 王双双[1] 姚玉新[1] 赵长增[2] 郝玉金[1] 由春香[1] 

机构地区：[1]山东农业大学作物生物学国家重点实验室,园艺科学与工程学院,山东泰安271018 [2]甘肃农业大学农学院园艺系,兰州730070

出　　处：《园艺学报》2009年第9期1255-1260,共6页Acta Horticulturae Sinica

基　　金：国家‘863’计划项目(2008AA10Z157);高等学校博士点基金项目(20060434009);山东省良种工程项目

摘　　要：通过RT-PCR扩增,从苹果叶片cDNA中克隆了FT基因的同源基因MdFT,构建了花椰菜病毒35S启动子驱动的MdFT植物表达载体35S::MdFT,并利用根癌农杆菌介导法将其导入番茄栽培品种‘中蔬四号’;同时转化拟南芥AtFT基因作为阳性对照。从添加卡那霉素的筛选培养基上再生了抗性植株,PCR扩增证明,外源基因MdFT和AtFT已经整合到转基因番茄的基因组,半定量RT-PCR则证明它们已经在转基因番茄中得到异位过量表达。形态鉴定发现,转基因番茄植株比非转基因对照植株开花早,表明成功地从苹果中克隆了成花素基因MdFT,该基因具有通过转基因缩短苹果树童期的潜在价值。It is of great importance for field yield of fruit trees to generate flower buds. Recently, FT protein has been proved to be a florigen in higher plants. In this study, full-length cDNA of MdFT gene, a homolog of Arabidopsis AtFT, was amplified from apple leaf cDNAs with RT-PCR. Subsequently, a plant expression construct containing MdFT gene driven by cauliflower mosaic virus 35S promoter was obtained and introduced into tomato cuhivar ＇Zhongshu 4＇ with Agrobacterium-mediated transformation. In parallel, Arabidopsis AtFT gene was transformed as a positive control. Finally, transgenic tomato lines were regenerated from selection medium plus kanalnycin. PCR amplification verified the integration of exogenous genes into the host genome, and semiquantitative RT-PCR displayed their ectopic overexpression in transgenic tomatoes. Furthermore, morphological observation found that transgenic tomato lines flowered earlier than the non- transgenic control, suggestive of our successful cloning of florigen gene MdFT from apple, which has the potential as a target gene to shorten juvenility in apple tree.

关 键 词：苹果 FT基因 异位过量表达 番茄 

分 类 号：S661.1[农业科学—果树学]