过氧化氢与蝴蝶兰胚性愈伤组织诱导  

H_2O_2 and Embryogenic Callus Induction of Phalaenopsis spp.

作　　者：刘福平[1] 陈淳[1] 许传俊[1] 

机构地区：[1]福建省亚热带植物研究所,福建厦门361006

出　　处：《园艺学报》2009年第9期1339-1344,共6页Acta Horticulturae Sinica

基　　金：福建省自然科学基金项目(C0410040)

摘　　要：从蝴蝶兰类原球茎切块诱导胚性愈伤组织,诱导期间培养物DNA甲基化程度持续下降,与H2O2含量变化呈极显著负相关。在降低6-BA浓度的培养基中添加H2O2可提高愈伤组织诱导率,在诱导培养基中添加H2O2淬灭剂二甲基硫脲降低了愈伤组织诱导率,H2O2可能是诱导胚性愈伤组织的信号分子之一。愈伤组织诱导期间SOD活性对HO水平变化起主导作用。Embryogenic callus was induced from PLB （protocorm-like body） cubes of Phalaenopsis spp. During callus induction, DNA methylation levels in cultures declined continuously and had a very significant negative correlation with the change of H2O2 content. Supplemented H2O2 in the medium containing lower concentration of growth regulator （6-BA） , the induction rate of callus increased, and this indicated that exogenous H2O2 could be used to replace part of growth regulator for embryogenic callus induction. The induction rate of embryogenic callus decreased when supplemented dimethylthiourea, a quencher of H2O2, in culture medium. H2O2 was possibly a signal molecule that mediated the induction of embryogenic callus. In callus induction period, SOD activity played an important role in the change of H2O2 content in cultures.

关 键 词：蝴蝶兰 胚性愈伤组织 H2O2 DNA甲基化 抗氧化酶 

分 类 号：S682.31[农业科学—观赏园艺] Q943.1[农业科学—园艺学]