急性冠状动脉综合征患者外周血单个核细胞核因子-κB蛋白表达水平与冠状动脉病变特征的关系  

The correlation between nuclear factor kappa B expressed in peripheral blood mononuclear cells and the characteristics of coronary atherosclerosis plaque in patients with acute coronary syndrome

作　　者：任法新[1] 方毅民[1] 杨军[1] 刘少荣[1] 张传焕[1] 

机构地区：[1]青岛大学医学院附属烟台毓璜顶医院心内科,264000

出　　处：《中华老年医学杂志》2009年第9期712-714,共3页Chinese Journal of Geriatrics

摘　　要：目的探讨核因子-κB（NF-κB）蛋白表达水平的变化与急性冠状动脉综合征（ACS）患者冠状动脉病变特征的关系。方法初步诊断为冠心病并行选择性冠状动脉造影（CAG）患者共81例。根据病情将患者分为ACS组50例，包括急性心肌梗死21例，不稳定型心绞痛29例；稳定型心绞痛（SAP）组31例；选择同期CAG正常者14例作为对照组。采用免疫印迹法检测外周血单个核细胞（PBMC）NF-κB蛋白表达水平的变化，并结合Gensini冠状动脉病变积分标准比较NF-κB蛋白表达水平与冠状动脉管腔病变程度的关系。结果ACS组NF-κB蛋白表达水平较SAP组和对照组明显增高，分别为0．85±0．18、0．75±0．21和0．71±0．23（F=3．72，P〈0．05）；NF-κB蛋白表达水平与Gensini冠状动脉病变积分无相关性（r=0．07，P〉0．05）。结论ACS患者存在NF-κB的激活，且NF-κB蛋白表达水平与冠状动脉病变狭窄程度无相关性。Objective To explore the correlation between expression of nuclear factor kappa B （NF-kappa B） in peripheral blood monouclear cells（PBMC） and the severity of coronary artery lesion in patients with acute coronary syndrome（ACS）. Methods The 81 patients were diagnosed with coronary heart disease, and all of them underwent immediate or selective coronary angiography（CAG）. The nuclear protein level of activated NF-κB was detected by Western blot and semi-quantity image analysis was done. Coronary angiograms were scored according to Gensini integration. The relationship between NF-κB and Gensini score was analyzed. Results The protein expression of NF-κB in PBMC in ACS group was significantly higher than that in stable angina group and control group （0.85±0.18 vs. 0.75±0.21 and0.71±0.23, F 3.72, P〈0.05）. The protein expression of NF-κB was not correlated with Gensini score（r=0.07 ,P〉0.05）. Conclusions The ACS patients have the activation of NF-κB in PBMC. The protein expression of NF-κB is not correlated with Gensini score, which does not suggest an implication of the severity of coronary artery.

关 键 词：NF-κB 冠状动脉疾病 炎症 冠状血管造影术 

分 类 号：R686[医药卫生—骨科学]