CCR5Δ32等位基因多态性与贵州特殊人群HBV感染的相关性  

Association of CCR5-Δ32 polymorphism with HBV infection in special population of Guizhou Province

作　　者：谭奎[1] 单可人[1] 何燕[1] 张婷[1] 李毅[1] 王婵娟[1] 齐晓岚[1] 赵艳[1] 肖雁[1] 谢渊[1] 吴昌学[1] 官志忠[1] 任锡麟[1] 

机构地区：[1]贵阳医学院分子生物重点实验室,贵州省贵阳市550004

出　　处：《世界华人消化杂志》2009年第22期2317-2319,共3页World Chinese Journal of Digestology

基　　金：贵州省科技厅科技计划课题基金资助项目;No.黔科合SY字[2008]3053;贵州省省长基金黔科教办基金资助项目;No.[2005]14;贵州省科技厅科研基金资助项目;No.黔科合J字[2005]2016号;贵州省科技厅科研基金资助项目;No.黔科合J字[2007]2032~~

摘　　要：目的:探讨CCR5Δ32等位基因在贵州世居少数民族(彝、瑶)与汉族人群的分布,并分析CCR5位点突变与HBV的关系.方法:采用PCR技术分别扩增贵州92份黔西彝族、101份威宁彝族、138份荔波瑶族以及165份毕节汉族CCR5编码区片段,通过琼脂糖凝胶电泳对CCR5Δ32多态性进行分析,最后抽取样本并进行DNA测序验证.结果:经过PCR特异性扩增,琼脂糖凝胶电泳后,496份样本均未发现CCR5Δ32突变型和杂合子.DNA测序验证所有样本CCR5基因未发生32碱基缺失的突变.结论:CCR5Δ32的分布有较明显的地域和种族差异,该位点与HBV感染的相关性有待于进一步深入研究.AIM： To investigate the distribution of CCR5-△32 polymorphism in Han nationality, Yi and Yao minorities of Guizhou and analyze the association between CCR5-△32 polymorphism and HBV infection. METHODS： Blood samples were taken from 92 Chinese Yi ethic individuals in Qianxi, 101 Chinese Yi ethic individuals in Weining, 138 Chinese Yao ethic individuals in Libo and 165 Chinese Han ethic individuals in Bijie. The coding region of the CCR5 gene was amplified by PCR and resolved by agarose gel electrophoresis to analyze the polymorphism of CCR5-△32. DNA sequencing was performed to verify the above results. RESULTS： The 32-base pair deletion allele in the CCR5 gene was not found in all 496 samples. DNA sequencing verified that no CCR5-△32 allele was detected in all samples. CONCLUSION： No CCR5-△32 allele is detected in this study in special population of Guizhou Province though the distribution of CCR5-△32 allele has geographical and ethnic differences.

关 键 词：趋化性细胞因子受体 多态性 世居少数民族 乙型肝炎病毒 感染 

分 类 号：R512.62[医药卫生—内科学]