碘克沙醇对体外血管内皮细胞的影响  

Effect of Iodixanol on vascular endothelial cell in vitro

作　　者：沈莉[1] 张建秋 滕志涛[1] 冯静波[1] 赵培勇[1] 周新福[1] 李尚艾[1] 李婧[1] 王金会[1] 孟子敏[1] 

机构地区：[1]威海市立医院心内科,山东威海264200 [2]威海市立第二医院电生理科,山东威海264200

出　　处：《中国介入影像与治疗学》2009年第5期399-401,共3页Chinese Journal of Interventional Imaging and Therapy

基　　金：威海市科技发展计划项目(2007GGA000041)

摘　　要：目的观察非离子型等渗对比剂碘克沙醇(威视派克)随浓度和时间变化对人血管内皮细胞的活力影响,探讨对比剂毒副作用发生的可能机制。方法人脐静脉血管内皮细胞株置于含不同浓度(4%、10%、20%)非离子型等渗对比剂碘克沙醇培养液中24、48、72 h后,通过噻唑蓝比色法检测细胞增殖活力,用Annexin V/PI双染色法进行细胞凋亡测定,观察不同条件下内皮细胞对对比剂的反应。结果碘克沙醇使血管内皮细胞吸光度下降(P<0.01);不同浓度影响不尽相同,而不同作用时间间差异无统计学意义(P>0.05)。碘克沙醇使血管内皮细胞凋亡率明显增高(P<0.05),不同浓度间差异无统计学意义(P>0.05)。结论体外培养环境下非离子型等渗对比剂碘克沙醇随浓度和时间变化会对内皮细胞产生活力影响,并诱导其凋亡。Objective To investigate the influence of different time and concentrations of non-ionic isotonic contrast media Iodixanol on the vitality of human vascular endothelial cell in vitro,and to explore the possible mechanism of contrast media side effect.Methods The human vascular endothelial cell was incubated in the 4%,10% or 20% non-ionic isotonic contrast media Iodixanol for 24,48 or 72 h.Cellular proliferative activity was detected with MTT colorimetric assay.Annexin V/PI double staining method was used to detect apoptosis.The response of endothelial cell to contrast agent in different conditions was observed.Results Iodixanol reduced the absorbance of human vascular endothelial cell（P〈0.01）,the impacts of different concentrations were not the same.No statistical significance was found among the impacts of different action time（P〉0.05）.Iodixanol increased apoptosis rate of human vascular endothelial cell（P〈0.05）.No statistical significance was found in different concentrations groups（P〉0.05）.Conclusion Iodixanol decreases the vitality of endothelial cell and increases apoptotic rate on different concentrations and different time in vitro.

关 键 词：等渗对比剂 碘克沙醇 血管内皮细胞 凋亡 

分 类 号：R543[医药卫生—心血管疾病]