HPLC测定复尔康注射液中维A酸在家兔体内的药动学  

STUDY ON PHARMACOKINETICS OF RETINOIC ACID OF FUERKANG INJECTION IN RABBIT BY HPLC

作　　者：李蓉[1] 肖顺汉[1] 冯文宇[2] 李茂[1] 任美萍[1] 刘明华[1] 

机构地区：[1]泸州医学院药理毒理学研究室,四川泸州646000 [2]泸州医学院药物研究所,四川泸州646000

出　　处：《泸州医学院学报》2009年第5期476-478,共3页Journal of Luzhou Medical College

基　　金：四川省教育厅青年基金项目(No.2006B025)

摘　　要：目的:采用高效液相色谱法(HPLC)测定复尔康注射液中维A酸的血药浓度,计算药动学参数,并与维A酸对照品溶液比较其药动学特征。方法:色谱柱:Dikma Kromasil C18(250mm×4.6mm,5μm);流动相:乙腈-0.1%磷酸溶液;流速:1.0ml·min-1;检测波长:355nm;柱温:30℃;进样量:20μl。结果:复尔康注射液中维A酸和维A酸对照品溶液在家兔体内均符合开放二室动力学模型特征。复尔康注射液中维A酸的t1/2α=10.61min,t1/2β=99.44min,K10=0.022min-1,AUC=257.15μg·min·ml-1,CL=0.022mg·kg-1·min-1;维A酸对照品溶液的t1/2α=8.61min,t1/2β=54.06min,K10=0.033min-1,AUC=174.87μg·min·ml-1,CL=0.032mg·kg-1·min-1。结论:与维A酸对照品溶液相比,复尔康注射液中维A酸的消除速率减慢,生物利用度增加,在体内滞留时间延长。Objective： To determine the plasma concentration of retinoic acid of Fuerkang injection by HPLC, calculate pharmacokinetics parameters and compare with reference retinoic acid solution. Methods：Column：Dikma Kromasil C18 （250 mm×4.6 mm, 5μm）;Mobile phase： acetonitrile- 0.1%phosphoric acid; Flow rate： 1.0 ml· min^-1; Detection wave length： 355nm;Column temperature：30℃ and sample size was 20μl. Results：Retinoic acid of Fuerkang injection and reference solution showed typical characteristics of open two-compartment dynamic model. Retinoic acid of Fuerkang injection： t1/2α=10.61min, t1/2β=99.44min, K10=0.022min^-1, AUC=257.15μg-min-ml^-1, CL= 0.022mg· kg^-1 min^-1.Reference retinoic acid solution：t1/2α=8.61min, t1/2β=54.06min, K10=0.033min^-1, AUC=174.87μg- min·ml^-1,CL=0.032mg ·kg^-1 ·min^-1. Conclusion： Retinoic acid of Fuerkang injection compared with reference retinoic acid solution, the rate of elimination was slowed, the bioavailability was increased and the retention time in the body was prolonged.

关 键 词：复尔康注射液 维A酸 药动学 高效液相色谱法 

分 类 号：R969.1[医药卫生—药理学]