清肺化痰、益气活血对肺纤维化大鼠不同时间点TGF-β_1 mRNA表达的影响  

Influences of therapies of clearing lung and dissolving phlegm,and replenishing qi and activating blood circulation on TGF-β_1 mRNA expression at different time points in rats with pulmonary fibrosis*

作　　者：翟华强[1] 刘洋[1] 姜楠[2] 

机构地区：[1]北京中医药大学中药学院,北京100102 [2]湖北中医学院附属医院

出　　处：《北京中医药大学学报》2009年第8期533-536,共4页Journal of Beijing University of Traditional Chinese Medicine

基　　金：教育部高等学校博士学科点专项科研基金资助项目(No.20070026025)

摘　　要：目的比较清肺化痰、益气活血中药对肺纤维化大鼠不同时间、不同吞噬细胞β型转化生长因子(TGF-β1)mRNA表达的影响。方法将77只雄性SPF级W istar大鼠随机分为7组,即假手术组、模型组、清肺化痰组、益气活血组、两法联合运用组、两法分阶段运用组及氢化可的松组,建立肺泡巨噬细胞(AM)、肺间质巨噬细胞(IM)细胞模型。用原位杂交方法检测TGF-β1mRNA表达水平,积分光密度法计算TGF-β1mRNA阳性面积。结果7 d时AM和28 d时IM的TGF-β1mRNA阳性细胞率和积分光密度显示,模型组较假手术组增加,差异具有极显著性(P<0.01);中药各治疗组较模型组降低,差异具有显著性(P<0.05)。结论清肺化痰、益气活血中药均可通过减少AM、IM分泌TGF-β1,抑制肺泡炎和肺纤维化的进程。Objective To compare the influences of the therapies of clearing lung and dissolving phlegm, and replenishing qi and activating blood circulation on the expressions of transform growth factor- β1 （TGF-β1） mRNA at different time points and in different phagocytes in rats with pulmonary fibrosis. Methods All male SPF Wistar rats （n =77） were randomly divided into 7 groups, including the shamoperation group （group 1 ）, model group （group 2）, group treated with the therapy of clearing lung and dissolving phlegm （group 3 ）, group with the theapy of replenishing qi and activating blood circulation （group 4）, group with combined therapy （group 5）, group with combined therapy in different stages （group 6） and group with hydroeortisone （group 7）. The model of alveolar macrophage （AM） and the model of interstitial macrophage （IM） were established. The expression of TGF-β1 mRNA was detected by using in situ hybridization, and the positive area of TGF-β1 mRNA was calculated by using integral optical density （IOD） method. Results The detective results of AM on 7th day and IM on 28th day howed that the positive cell rate and IOD of TGFβ1 mRNA increased in group 2 compared with those in group 1 with a great significant difference （p 〈 0.01 ）, while those in group 3, 4, 5 and 6 decreased ：ompared with those in grouo 2 with a significant difference （P 〈 0.05）. Conclusion The therapies of clearing lung and dissolving phlegm, and alveolitis and the development of pulmonary IM. tonifying qi and replenishing blood fibrosis through reducing the secretio circulation n of TGF-β1 can inhibit in AM and IM.

关 键 词：肺纤维化 清肺化痰 益气活血 肺泡巨噬细胞 肺间质巨噬细胞 β型转化生长因子 大鼠 

分 类 号：R285.5[医药卫生—中药学]