免疫荧光磁粒分离诱导CD34^+细胞转化为DC的实验研究  

Application of Immunologic Nano-Fluorescence Beads in Separation of CD34^+ Cells into Dendritic Cells

作　　者：季倩[1] 李兴玉[1] 杨兵[1] 

机构地区：[1]上海师范大学生命与环境科学学院,上海200234

出　　处：《现代生物医学进展》2009年第16期3041-3045,F0003,共6页Progress in Modern Biomedicine

基　　金：上海师范大学大学生科技创新项目(CH031)

摘　　要：目的:建立一种磁荧光分离细胞的方法将CD34+细胞转化为DC。方法:人脐带血单个核细胞,经免疫免疫荧光标记、外磁场分离CD34+细胞,分别加入GM-CSF、IL-4和不同浓度黄芪多糖,诱导12-14天检测DC细胞数量和功能。结果:随黄芪多糖浓度的升高,DC细胞的百分率越大。结论:黄芪多糖可作为一种优良的诱导促进剂,联合使用GM-CSF和IL-4可将CD34+细胞转化成为DC。Objective： To establish a reliable method of separation of cells by magnetic fluorescence beads, and to provide an excellent inducer （Astragalus Polyose, APS） of dendritic cells. Methods： Umbilical cord blood was collected to separate CD34＋ cells in the external magnetic field conditions by magnetic fluorescence beads which been marked, then induced by GM-CSF, IL-4 and different con- centrations ofAstragalus Polysaccharide for 12-14 days. The DC cells number and function was tested after induction, Result： The percentage of DCs increased with the increases of the APS concentration. Conclusion： DCs can be induced from CD34＋ cells with APS, GM-CSF and IL-4, and APS could be the excellent inducer of dendritic cells.

关 键 词：抗原递呈细胞 树突状细胞 自然杀伤细胞 黄芪多糖 

分 类 号：Q291[生物学—细胞生物学] Q343