1株来自陕西盐湖的极端嗜盐古菌的分离及鉴定  

Isolation and Identification of an Archaeon Isolated from a Saline Lake in Shaanxi Province

作　　者：赵萍[1] 邹正中[1] 王革娇[1] 

机构地区：[1]华中农业大学生命科学技术学院/农业微生物学国家重点实验室,武汉430070

出　　处：《华中农业大学学报》2009年第5期573-576,共4页Journal of Huazhong Agricultural University

基　　金：国家自然科学基金项目(30671140);教育部博士点基金项目(20060504027)资助

摘　　要：从陕西榆林苟池盐湖水中分离到1株嗜盐古菌GS1并对其进行了系统鉴定。通过形态鉴定、革兰氏染色、生长盐浓度试验、Mg2+浓度试验、生长pH范围、生理生化鉴定、16S rRNA基因序列等分析,确认该菌为1株新型的极端嗜盐古菌,属于盐红菌属(Halorubrum)。GS1与Halorubrum saccharovorum(U17346)的16S rRNA基因序列同源性为97%。命名为Halorubrumsp.GS1。其16S rRNA基因序列已被GenBank数据库收录,序列号为FJ793266。A halophileic archaeon strain GS1 was isolated from a salt lake water located in Yulin city,Shaanxi Province. A series of tests were performed for strain identification including morphology characterization, Gram staining, NaCl range for growth, Mg^2＋ range for growth and pH range, physiological/biochemical and 16S rRNA gene analyses. The results indicated that GS1 was a novel extreme halophilic archaeon strain belonging to genus Halorubrum. The 16S rRNA gene sequence similarity between strains GS1 and Halorubrum saccharovorurn （U17346） was 97%. This strain is named as Halorubrurn sp. GS1. The GenBank accession number of its 16S rRNA gene sequence is FJ793266.

关 键 词：极端嗜盐古菌 盐红菌属 盐湖 陕西省 16S RRNA基因 

分 类 号：Q939.9[生物学—微生物学]