猪繁殖与呼吸综合征病毒分离株GP5基因的遗传变异与系统进化分析  

Genetic Variation and Phylogenetic Analysis of Glycoprotein 5 of PRRSV

作　　者：梁望旺[1,2] 杨克礼[1] 伍锐[1] 熊忠良[1] 刘泽文[1] 段正赢[1] 徐涤平[1] 

机构地区：[1]湖北省农科院畜牧兽医研究所,武汉430064 [2]动物胚胎工程及分子育种湖北省重点实验室,武汉430064

出　　处：《华中农业大学学报》2009年第5期595-599,共5页Journal of Huazhong Agricultural University

基　　金：国家科技支撑计划(2006BAD06A18);湖北省重点实验室开放课题(2007ZD08)资助

摘　　要：为进一步了解猪繁殖与呼吸综合征病毒（PRRSV）在国内的分子遗传进化情况，对中国大陆1996-2008年问33株PRRSV分离毒株（29株来自GenBank）的GP5序列与国外部分代表毒株进行了分析比较。结果表明，所选PRRSV中国分离毒株中有32株属于北美洲型（NA），并大致可分为2个基因亚群，1株属于欧洲型（EU）。其中，自2006年在我国暴发的高致病性蓝耳病病毒（H—PRRSV）属于亚群1，且与YA株亲缘关系较近；亚群2毒株则与Ingelvac的弱毒疫苗株及其亲本株VR-2332具有较高的同源性。An outbreak of highly virulent porcine reproductive and respiratory syndrome virus （H- PRRSV） in most areas of China recently has led to huge economic loss. To more fully understand the extent of genetic diversity of PRRSV, we analyzed and compared the GP5 sequence of 33 PRRSV isolates （29 derivated from GenBank） from 1996 to 2008 in China's Mainland. Sequence analysis revealed that the 32 Chinese isolates studied belong to the NA-type and could be classified into two subgroups on the genotype phylogenetic tree. We found that the H PRRSV isolates belonged to subgroup 1 and shared a high identity with PRRSV YA. The subgroup 2 isolates were more closely related to Ingelvac PRRS MLV vaccine virus and its parent virus VR-2332.

关 键 词：猪繁殖与呼吸综合征病毒 进化分析 GP5 ORF5 

分 类 号：S852.65[农业科学—基础兽医学]