Cr^6＋胁迫对高丹草幼苗根质膜氧化及ATPase活性的影响  

Effects of Cr^(6+) on lipid peroxidation and ATPase activity of Sorghum Hybrid Sudangrass (Sorghum bicolor×S. Sudanense) roots

作　　者：赵晖[1] 吕金印[1] 张素娜[2] 侯晓林[1] 

机构地区：[1]西北农林科技大学,陕西杨凌712100 [2]西北农林科技大学理学院,陕西杨凌712100

出　　处：《西北农林科技大学学报（自然科学版）》2009年第10期167-172,共6页Journal of Northwest A&F University(Natural Science Edition)

基　　金：农业部农业公益性行业科研专项“核技术农业应用”(200803034)

摘　　要：【目的】研究不同浓度Cr6+处理对高丹草幼苗根质膜氧化及ATPase活性的影响,阐明重金属Cr对植物的毒害机理,为铬污染地区优质牧草的种植提供理论依据。【方法】采用不同浓度(0(对照),0.5,1.0,1.8mmol/L)K2CrO4胁迫处理高丹草幼苗,测定根长、根干质量、根系中的Cr含量、根系O2.-产生速率和H2O2、丙二醛(MDA)含量及根质膜H+-ATPase、Ca2+-ATPase活性的变化。【结果】与对照相比,随着Cr6+处理浓度的增加,高丹草幼苗根长和根干质量均呈先升高后降低的变化趋势,O2.-产生速率、H2O2和MDA含量及细胞质膜相对透性显著升高(P<0.05),且与Cr6+处理浓度呈正相关。0.5 mmol/L Cr6+浓度处理时,H+-ATPase、Ca2+-ATPase活性与对照差异不显著(P>0.05),而在1.0,1.8 mmol/L Cr6+处理时,ATPase活性显著降低(P<0.05),并且与膜脂过氧化水平呈负相关。【结论】低浓度Cr6+处理对高丹草幼苗根系质膜ATPase活性影响较小,较高浓度Cr6+处理使AT-Pase活性及根生长受到抑制。[Objective] The research investigated the effects of Cr^6＋ on lipid peroxidation and ATPase activity of Sorghum Hybrid Sudangrass （Sorghum bicolor × S. Sudanense） seedling roots,and explored the toxic hazard of heavy metal Cr Stress on Plant. [Method] The effects of different concentrations of Cr^6＋ （0 （CK）, 0.5,1.0,1.8 mmol/L K2 CrO4 ） on the length and dry weight of roots, generating rate of O^.- , H2O2 and MDA content as well as plasma membrane relative permeability, activities of H^＋-ATPase, Ca^2＋-AT- Pase in the plasma membrane of Sorghum Hybrid Sudangrass seedlings roots were investigated. [Result] Compared with the control, the length and dry weight of roots increased first and then decreased with the increase of concentrations of Cr^6＋ ,which resulted in significant increase of O^.- and H2O2 level and malondialdehyde（MDA） content as well as plasma membrane relative permeability in the roots（P〈0.05）, and there was significantly positive correlation to Cr^6＋ treatment concentration. Moreover, the activity of H^＋-ATPase and Ca^2＋-ATPase had no obvious change（P〉0.05）under 0.5 mmol/L Cr^6＋ concentration, but significantly decreased with 1.0,1.8 mmol/L Cr^6＋ stress（P〈0.05）, a negative correlation was present between the level of membrance-lipid and the two enzyme. [Conclusion] We concluded that there was unnoticeable change under low concentration of the Cr^6＋ in the activity of ATPase in Sorghum Hybrid Sudangrass seedlings roots plasma membrane, whereas it would dropped markedly and the root growth was repressed under relatively high Cr^6＋ stress.

关 键 词：高丹草 铬污染 膜脂过氧化 H^+-ATPASE CA^2+-ATPASE 

分 类 号：S544.044[农业科学—作物学] X503.233[环境科学与工程—环境工程]