不同倍性水稻植株茎解剖结构比较研究  

Comparative studies on stem anatomical structure of rice plants with various ploidy level

作　　者：金亮[1] 薛庆中[1] 肖建富[1] 张宪银[1] 

机构地区：[1]浙江大学农业与生物技术学院农学系,浙江杭州310029

出　　处：《浙江大学学报（农业与生命科学版）》2009年第5期489-496,共8页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：国家自然科学基金资助项目(30571146);浙江省水稻攻关资助项目(04-06)

摘　　要：为研究倍性变化对水稻植株茎结构的影响,以粳稻FC311和FC322为供试品系,应用流式细胞术从花培后代中鉴定出6份不同倍性的材料(FC311中的单倍体、二倍体及三倍体;FC322中的单倍体、二倍体及四倍体),利用石蜡切片法观察并比较不同倍性植株茎的解剖结构.结果表明:供试材料节间维管束数及大小、茎壁面积、维管束面积通常与倍性的增加有关,显示明显的基因剂量效应;而表皮下和内、外圈维管束鞘的厚壁细胞层数、发育特征及细胞淀粉粒含量等结构和特性,与倍性变化无明显相关;在FC322四倍体植株第1节间中,还观察到颇为少见的2层厚壁组织结构.To investigate the effects of ploidy changes on the stem structure, by using flow cytometry （FCM）, we distinguished six different ploidy materials （haploid, diploid, and triploid of line FC311 and haploid, diploid, and tetraploid of line FC322）, in rice （Oryza sativa L. subsp. Japonica）. Also, we compared the anatomical stern structure of various ploidy rice plants with paraffin sections. The results show that the increase of the number and size of vascular bundle, and area of stem wall and vascular bundle in internodes were frequently associated with increase in ploidy level of the tested plants, indicating a significant gene dosage effect. However, there were no obvious correlations between different ploidy levels and the following traits, such as, layer number and development of collenchyma cell under epiderm in vascular bundle sheath of outer circle and inner circle, starch grains in ground tissue cells, the ratio of total area of vascular bundle and area of stem wall. The structure of two layers of collenchyma cell was rarely observed in the 1st internode of FC322 tetraploid plant.

关 键 词：水稻 倍性 流式细胞术 茎 解剖结构 

分 类 号：Q944.5[生物学—植物学]