腊样芽孢杆菌PAS38和甘露聚糖制剂对家兔小肠SS及5-HT免疫阳性细胞的影响  

Effect of Bacillus cereus PAS38 and mannosan preparation on immunoreactive cells of somatostatin and 5-HT in small intestine of rabbit

作　　者：潘康成[1] 陈正礼[1] 崔恒敏[1] 袁朝富[1] 陈刚[1] 

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,四川雅安625014

出　　处：《浙江大学学报（农业与生命科学版）》2009年第5期578-584,共7页Journal of Zhejiang University:Agriculture and Life Sciences

基　　金：四川省重点建设学科资助项目(SZD0418);教育部长江学者和创新团队发展计划"创新团队资助项目(IRT0848)

摘　　要：选用60只雄性断奶仔兔随机分为4组,对照组饲喂基础日粮,PAS38组在基础日粮中添加0.1%腊样芽孢杆菌PAS38制剂,甘露聚糖组在基础日粮中添加0.2%β-甘露聚糖制剂,菌糖混合组在基础日粮中分别添加0.1%PAS38制剂和0.2%β-甘露聚糖制剂.家兔饲喂至第15、30、45天时每组宰杀5只,采用免疫组织化学SABC法检测家兔小肠中生长抑素(SS)、5-羟色胺(5-HT)阳性细胞数量和表达.结果表明:PAS38制剂和甘露聚糖制剂均减少家兔小肠中SS阳性细胞的数量和降低SS细胞的表达强度,增加小肠中5-HT阳性细胞数量和提高5-HT细胞的表达强度;两者联合应用效果更加明显,与对照组相比具有显著或极显著差异(P<0.05或P<0.01),表现出一定的协同效应.Sixty male weaned rabbits were randomly divided into four groups： control group was fed the basic diet ; PAS38 group was fed diet supplemented with O. 1 % B. cereus PAS38 ; mannosan group was fed diet supplemented with 0.2% mannosan; and mix group was fed diet supplemented with 0.1% B. cereus PAS38 and 0. 2% mannosan. Immunohistochemistry SABC was used to measure 5-HT and somatostatin （SS） of immunoreactive cells and the expression in small intestine of rabbit on the 15, 30 and 45th day. The results show that the additive of PAS38 or β-mannan could decrease the number of immunoreactive cells of SS and the expression of SS, and increase the number of immunoreactive cells of 5-HT and the expression of 5-HT in small intestine of rabbit. United application of two kinds of additives showed obviously efficacious （P〈 0.05 or P〈 0.01）, compared with control group. The results above indicated that combined application of B. cereus PAS38 and β--mannan preparation could achieve good synergistic effect on modulating intestinal endocrine-immunity of rabbit.

关 键 词：微生态制剂 生长抑素 5-羟色胺 免疫组织化学 小肠 

分 类 号：S816.7[农业科学—饲料科学]