3-甲磺酰基丙基异硫代氰酸酯的提取分离与抗肿瘤的体外试验  

Studies on Purification and Antitumor in Vitro Experiment of Iberin

作　　者：王伟[1] 苏光耀[1] 沈莲清[1] 

机构地区：[1]浙江工商大学食品与生物工程学院,浙江杭州310035

出　　处：《食品与生物技术学报》2009年第5期637-641,共5页Journal of Food Science and Biotechnology

基　　金：浙江省政府重大科技专项招标项目(2004C12005)

摘　　要：为了得到具有抗癌有活性的、较高纯度的3-甲磺酰基丙基异硫代氰酸酯(iberin),作者研究了iberin的提取、分离及纯化工艺。确定了以丙酮为溶剂,从甘蓝种子的硫苷酶解产物中超声提取iberin粗提物,并用GC-MS鉴定其结构,再依次通过正相硅胶柱与Sephadex LH-20凝胶柱分离提纯得到iberin样品,HPLC测定其纯度为80.9%。采用体外细胞筛选试验研究iberin的抗肿瘤活性,结果显示iberin对小鼠白血病细胞P388和人鼻咽癌细胞CNE均有很强的抑制效果。In order to obtain anticancer and high purified 3-methylsulpropane isothiocyanate （iberin）, the extraction, separation and purification of iberin obtained from enzymatic hydrolysates of glucosinolates in cabbage seed were carefully investigated . The iberin was ultrasonic extracted from cabbage seed with acetone and then determined by GC-MS. The extract were separated and purified by using the silica gel column chromatography and Sephadex LH-20 column chromatography. The purity of iberin was 80.9%, which was determined by high performance liquid chromatograph （HPLC）. Moreover, the antitumor effects of iberin were also tested. The results present here demonstrated that iberin exhibited a higher inhibition effect on the proliferation of P388 and CNE tumor cell.

关 键 词：甘蓝种子 异硫代氰酸酯 Iberin 提取分离 抗肿瘤活性 

分 类 号：R284.1[医药卫生—中药学] R285[医药卫生—中医学]