接种方式对阿维菌素发酵过程的影响  

Effect of Inoculation Methods on Avermectin Fermentation by Streptomyces avermilitis

作　　者：姚小员[1] 储消和 庄英萍[1] 梁剑光[1] 王永红[1] 储炬[1] 张嗣良[1] 

机构地区：[1]生物反应器工程国家重点实验室,华东理工大学,上海200237 [2]浙江省升华拜克生物股份公司,浙江湖州313200

出　　处：《食品与生物技术学报》2009年第5期682-687,共6页Journal of Food Science and Biotechnology

基　　金：国家“十一五”科技支撑计划项目(2008BAI63B01)

摘　　要：在摇瓶发酵中对除虫链霉菌的孢子接种工艺进行了初步研究，同时比较了孢子接种、常规的挖块接种和菌丝接种3种不同接种方式对除虫链霉菌菌体生长和细胞代谢参数的影响。实验结果表明：孢子接种的最适孢子接种量在4～6×10^6个／mL范围内，而不同接种方式导致了发酵过程除虫链霉菌营养物质消耗、PH值变化、菌丝形态以及效价等方面差异。孢子接种方式在摇瓶发酵中表现出明显优势，采用孢子接种方式获得最终阿维菌素的效价比常规的挖块接种方式提高10％以上，比菌丝接种方式提高了20％以上。The spore inoculation technology of S. averrnitilis was examined in shake flask culture and the effect of three inoculation methods, spore inoculation, block dug inoculation and mycelia inoculation, on the growth and metabolism of S. avermitilis were investigated. The results showed that the optimum spore inoculum level was ranging between 4 × 10^6 and 6 × 10^6 spores/ mL, the nutrition consumption, pH, the morphology of S. avermitilis and the yield of avermectin during fermentation process were different. By the spore inoculation, the yield of avermectin increased by 10% and 20% as compared with that of the block dug inoculation and mycelia inoculation, respectively. In conclusion, the method of spore inoculation has obvious effect on S. avermitilis growth and metabolites production.

关 键 词：除虫链霉菌 阿维菌素 孢子接种 接种方式 

分 类 号：S482.2[农业科学—农药学] S482.3[农业科学—植物保护]