哮喘患者血清中IL-4，IL-12，IL-13，IFN-γ，IgE水平的测定及其临床意义  

Changes of Serum IL-4,IL-12,IL-13,IFN-γ and IgE Levels and Their Clinical Significance in Acute Attack of Asthma

作　　者：黄永富[1] 余世庆[2] 杭杲[3] 徐永建[1] 

机构地区：[1]扬州大学第四临床医学院江苏南通瑞慈医院检验医学中心,江苏南通226010 [2]扬州大学第四临床医学院江苏南通瑞慈医院呼吸内科,江苏南通226010 [3]扬州大学第四临床医学院江苏南通瑞慈医院瑞慈儿童医院内科,江苏南通226010

出　　处：《现代检验医学杂志》2009年第5期120-123,共4页Journal of Modern Laboratory Medicine

基　　金：江苏省南通市社会发展科技基金资助项目（No.S5014）.

摘　　要：目的探讨哮喘发作期和缓解期血清白细胞介素-4（IL-4）、白细胞介素-12（IL-12）、白细胞介素-13（IL-13）和干扰素（IFN-γ）与免疫球蛋白E（IgE）的浓度变化及其意义。方法采用生物素亲合素双抗体夹心和间接ELISA法分别对60例哮喘急性发作期和缓解期患者进行血清IL-4，IL-12，IL-13，IFN-γ和IgE水平的测定。结果60例哮喘急性发作期和哮喘缓解期患者血清IL-4，IL-13水平均高于正常对照组（P〈0．01）；而血清IFN-γ，IL-12水平均较对照组（40例）显著下降（P〈0．01）；哮喘急性发作期患者血清IL-4，IL-13水平高于哮喘缓解期患者（P〈0．01），而IFN-γ，IL-12水平均较缓解组显著下降（P〈0．01）；在哮喘发作期患者血清中IL-4，IL-13水平与IgE含量呈显著正相关，而IL-12，IFN-γ水平与IgE呈显著负相关。结论细胞因子分泌紊乱、网络失衡是哮喘病急性发作的重要因素，而细胞因子IL-4，IFN-γ，IL-12，IL-13等在其发生、发展中起着重要的调控作用。Objective To explore the changes of serum IL-4,IL-12,IL-13,IFN-γ and IgE levels and their clinical significance during the acute attack and its catabasis of the patients with asthma. Methods Serum levels of IL-4,IL-12,IL-13, IFN-γ and IgE were estimated by double-antibody sandwich and indirect ELISA respectively in 60 eases of patients with acute asthmatic attack and its catabasis. Results The average levels of serum IL-4 and IL-13 respectively in 60 patients with asthma and its catabasis were significantly higher than those in control group（P〈0.01） ;their average levels of serum IL-12 and IFN-γ decreased significantly as compared with those in control group（P〈0.01）. Similarly,the average levels of serum IL-4 and IL-13 in patients with asthma were significantly higher than those with its catabasis （P〈0. 01）;whereas their average levels of serum IL-12 and IFN-γ decreased significantly as compared with the group of those in its catabasis （P〈0. 01）. A distinct positive correlation existed between the levels of serum IL-4 or IL-13 and those of IgE during the period of asthmatic attack and while a prominent negative correlation was found between the levels of serum IL-12 or IFN-γ and those of IgE. Conclusion The secretory disturbance of cell factors and balancing disorder of immune network are the important factors of acute asthmatic attack,IL-4,IL-12,IL-13 and IFN-γ play crucial roles of regulation in the occurrenee and development of this disease.

关 键 词：哮喘 白细胞介素4 白细胞介素12 白细胞介素13 γ干扰素 免疫球蛋白E 

分 类 号：R562.25[医药卫生—呼吸系统] R392.11[医药卫生—内科学]