壳聚糖-明胶/APTES改性生物活性玻璃复合支架的制备工艺  

Preparation of the porous scaffolds of chitosan-gelatin/APTES modified bioglass

作　　者：任力[1,2] 王迎军[1,2] 陈晓峰[1,2] 赵营刚[1] 

机构地区：[1]华南理工大学特种功能材料教育部重点实验室,广州510640 [2]华南理工大学材料学院,广州510640

出　　处：《复合材料学报》2009年第4期47-52,共6页Acta Materiae Compositae Sinica

基　　金：国家自然科学基金(50803018;50732003;50830101;U0834003);国家973项目(2005CB623902);广东省自然科学基金(04205786)

摘　　要：为改善生物活性玻璃与高分子之间的相容性,利用APTES改性生物活性玻璃(SBG),通过冷冻干燥法制备出用于骨和软骨组织工程的壳聚糖-明胶/APTES改性生物活性玻璃(CS-Gel/SBG)仿生型复合多孔支架,并对其孔隙率、力学性能和显微形貌进行了表征;探讨了各组分不同含量、交联剂和冷冻温度对CS-Gel/SBG复合支架孔隙率、力学性能和显微观结构的影响。研究表明,当SBG和CS-Gel的含量分别为70和40 g.L-1,用EDC和NHS交联,-50℃急冻2 h后,又在-15℃下冷冻10 h,最后真空冷冻干燥,制备出孔隙分布均匀、孔隙率达到90%以上、三维连通的复合多孔支架。A new kind of porous composite used as bone and cartilage tissue engineering scaffolds was prepared with chitosan, gelatin and 3-aminopropyltriethoxysilane modified bioglass（SBG） by the freeze-drying technique in order to improve the compatibility of BG and polymer. The porosity, bending strength and microstructure of the composite scaffolds were characterized. The effects of the concentration of SBG and CS- Gel, the cross linking agent and the super-cooling temperature on the microstructure of the composite scaffolds were investigated, respectively. The results show that the composite scaffolds with 3D interconnective porous structure, high porosity （90%） and high mechanical strength could be prepared under 70 g· L^-1 of SBG concentration and 40 g· L^-1 of CS- Gel concentration, then crosslinked with EDC and NHS, frozen at -50℃ for 2 h and --15℃ for 15 h, and finally lyophilized.

关 键 词：明胶 壳聚糖 生物活性玻璃 硅烷偶联剂 

分 类 号：TB332[一般工业技术—材料科学与工程]